Stimulation of the rat dentine-pulp complex by bone morphogenetic protein-7 in vitro
Stimulation of the rat dentine-pulp complex by bone morphogenetic protein-7 in vitro
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DOI：
10.1016/s0003-9969(99)00131-4
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发表时间：
2000-02-01
影响因子：
3
通讯作者：
Smith, AJ
中科院分区：
文献类型：
--
作者：
Sloan, AJ;Rutherford, RB;Smith, AJ
当将人重组骨形态发生蛋白-7（BMP-7）应用于猴牙齿中新切割的牙本质时，刺激了第三级牙本质的形成，但尚不清楚这种反应是否涉及上调现有成牙本质细胞的合成和分泌活性，或诱导新的成牙本质细胞样细胞的分化。使用最近开发的全牙切片器官培养系统，目的是在没有组织损伤的情况下检查 BMP-7 对现有成牙本质细胞的刺激和调节的影响。将琼脂糖珠浸泡在 500 ng/ml 或 100 ng/ml BMP-7 培养基溶液中，并置于大鼠牙齿切片的牙本质-牙髓复合物的成牙本质细胞区域。将切片包埋在半固体琼脂培养基中并在液-气界面培养7天。结果显示，浸泡在 500 ng/ml BMP-7 中的珠子刺激了施用部位的成牙本质细胞的细胞外基质分泌的局部增加，其刺激效果比较低浓度更大。这些作用对于牙本质-牙髓复合体内组织损伤后的修复过程可能很重要，并且可能有助于治疗性诱导第三期牙本质发生。 (C) 2000 Elsevier Science Ltd. 保留所有权利。
Human recombinant bone morphogenetic protein-7 (BMP-7), when applied to freshly cut dentine in monkey teeth, stimulated tertiary dentine formation, but it is unclear whether this response involved upregulation of the synthetic and secretory activity of existing odontoblasts or the induction of differentiation of new odontoblast-like cells. Using a recently developed organ-culture system for whole tooth slices, the aim here was to examine the effects of BMP-7 on the stimulation and modulation of existing odontoblasts in the absence of tissue injury. Agarose beads were soaked in a 500 ng/ml or 100 ng/ml solution of BMP-7 in culture medium and placed on the odontoblast area of the dentine-pulp complex of rat tooth slices. The slices were embedded in a semisolid agar-based medium and cultured at the liquid-gas interface for 7 days. Results showed that beads soaked in 500 ng/ml BMP-7 stimulated a localized increase in extracellular matrix secretion by odontoblasts at the site of application, with greater stimulatory effects than from the lower concentration. These effects may be important in the reparative processes after tissue injury within the dentine-pulp complex and may be useful in the therapeutic induction of tertiary dentinogenesis. (C) 2000 Elsevier Science Ltd. All rights reserved.