Effects of vitamin E and α-lipoic acid on skeletal muscle contractile properties
Effects of vitamin E and α-lipoic acid on skeletal muscle contractile properties
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DOI：
10.1152/jappl.2001.90.4.1424
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发表时间：
2001-04-01
影响因子：
3.3
通讯作者：
Packer, L
中科院分区：
文献类型：
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作者：
Coombes, JS;Powers, SK;Packer, L
使用原位大鼠胫骨前肌（TA）肌肉制备进行初步实验，以评估膳食抗氧化剂对肌肉收缩特性的影响。将成年Sprague-Dawley大鼠分成两个饮食组：1）对照饮食（Con）和2）补充维生素E（VE）和α-硫辛酸（α-LA）（Antiox）。Antiox大鼠用Con大鼠的饮食（AIN-93 M）与另外的10，000 IU VE/kg饮食和1.65g/kg α-LA喂养.经过8周的喂养期，两个饮食组之间的最大比张力在疲劳协议之前或之后或在疲劳协议期间的力产生没有差异（P > 0.05）。然而，在未疲劳的肌肉，最大的抽搐张力和强直性生产力在刺激频率小于或等于40 Hz的抗氧化剂的动物相比，与对照组（P < 0.05）。调查抗氧化剂是负责任的压抑力的生产，第二个实验进行了使用在体外大鼠膈肌制备。不同浓度的VE和二氢硫辛酸，减少形式的-LA，单独或组合添加到浴含有膈肌肌条。从这些实验的结果表明，高水平的VE抑制骨骼肌的生产力在低刺激频率。
Initial experiments were conducted using an in situ rat tibialis anterior (TA) muscle preparation to assess the influence of dietary antioxidants on muscle contractile properties. Adult Sprague-Dawley rats were divided into two dietary groups: 1) control diet (Con) and 2) supplemented with vitamin E (VE) and alpha -lipoic acid (alpha -LA) (Antiox). Antiox rats were fed the Con rats' diet (AIN-93M) with an additional 10,000 IU VE/kg diet and 1.65 g/kg alpha -LA. After an 8-wk feeding period, no differences existed (P > 0.05) between the two dietary groups in maximum specific tension before or after a fatigue protocol or in force production during the fatigue protocol. However, in unfatigued muscle, maximal twitch tension and tetanic force production at stimulation frequencies less than or equal to 40 Hz were less (P < 0.05) in Antiox animals compared with Con. To investigate which antioxidant was responsible for the depressed force production, a second experiment was conducted using an in vitro rat diaphragm preparation. Varying concentrations of VE and dihydrolipoic acid, the reduced form of -LA, were added either individually or in combination to baths containing diaphragm muscle strips. The results from these experiments indicate that high levels of VE depress skeletal muscle force production at low stimulation frequencies.