Rhoptry secretion of membranous whorls by Plasmodium falciparum merozoites.
Rhoptry secretion of membranous whorls by Plasmodium falciparum merozoites.
复制标题
恶性疟原虫裂殖子分泌膜轮的棒状体。
DOI：
10.4269/ajtmh.1986.35.37
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
J. Vanderberg
中科院分区：
文献类型：
--
作者：
M. J. Stewart;S. Schulman;J. Vanderberg
多层膜轮局部化，通过电子显微镜，从戊二醛单宁酸（TA）固定的裂殖子的人类疟疾寄生虫，恶性疟原虫的棒状体微线复合物的元素。这些多层结构，其中有一个暗线到暗线周期约为5 nm，也被发现在核膜和紧密贴壁裂殖子的外表面。含有胞内裂殖子的分节孢子囊泡常在其吸寄泡内呈膜涡状，并与吸寄泡膜的外表面紧密贴壁。在滋养体、未成熟的滋养体和未感染的红细胞中未发现轮状体。大多数棒状体的裂殖子固定在戊二醛-TA是电子透明的，而棒状体固定在戊二醛单独的电子致密。一些裂殖子固定在戊二醛-TA既有电子致密和电子透明的棒状体。这些结果表明，TA诱导棒状材料的过早挤出。我们的研究结果支持以前的建议，在文献中的裂殖子棒状体分泌的磷脂物质参与裂殖子与宿主红细胞的相互作用。
Multilamellar membranous whorls were localized, by electron microscopy, in elements of the rhoptry-microneme complex from glutaraldehyde-tannic acid (TA)-fixed merozoites of the human malarial parasite, Plasmodium falciparum. These multilaminate structures, which have a dark line-to-dark line periodicity of approximately 5 nm, were also found in the nuclear envelope and closely apposed to the external surface of merozoites. Segmented schizonts, which contain intracellular merozoites, often showed membranous whorls within their parasitophorous vacuoles and closely apposed to the external surface of the parasitophorous vacuole membrane. Whorls were not found in trophozoites, immature schizonts, and uninfected erythrocytes. Most rhoptries in merozoites fixed in glutaraldehyde-TA were electron-lucent whereas rhoptries fixed in glutaraldehyde alone were electron-dense. Some merozoites fixed in glutaraldehyde-TA had both an electron-dense and an electron-lucent rhoptry. These findings suggest that TA induces the premature extrusion of rhoptry materials. Our findings support previous suggestions in the literature that phospholipid materials secreted from merozoite rhoptries are involved in merozoite interaction with host erythrocytes.