Inhibition of Pim-1 attenuates the proliferation and migration in nasopharyngeal carcinoma cells
Inhibition of Pim-1 attenuates the proliferation and migration in nasopharyngeal carcinoma cells
复制标题
抑制 Pim-1 可减弱鼻咽癌细胞的增殖和迁移。
DOI：
10.1016/s1995-7645(12)60132-1
复制
发表时间：
2012-08-01
影响因子：
3.1
通讯作者：
Shen, Zhi-Hua
中科院分区：
文献类型：
--
作者：
Jie, Wei;He, Qi-Yi;Shen, Zhi-Hua
目的：探讨原癌基因Pim-1在鼻咽癌细胞增殖和迁移中的作用。研究方法：采用RT-PCR、Western blotting和免疫荧光法检测Pim-1在鼻咽癌细胞系CNE 1、CNE 1-GL、CNE-2 Z和C666-1中的表达。用不同浓度的Pim-1特异性抑制剂槲皮素处理CNE 1、CNE 1-GL和C666-1细胞后，分析细胞活力、集落形成率和迁移能力。结果如下：Pim-1在高分化的CNE 1细胞中呈阴性表达，在低分化的CNE-2 Z细胞中呈弱阳性表达，在未分化的C666-1细胞中呈强阳性表达。有趣的是，来自用Epstein巴尔病毒潜伏膜蛋白-1过表达质粒转染的CNE 1的CNE 1-GL细胞显示出更强的Pim-1表达。槲皮素处理CNE 1-GL和C666-1细胞后，细胞活力、集落形成率和迁移能力明显下降，但对CNE 1细胞无明显影响。结论：这些结果表明Pim-1过表达有助于NPC的增殖和迁移，靶向Pim-1可能是抗Pim-1表达的NPC的潜在治疗方法。
Objective: To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma (NPC) cells. Methods: Pim-1 expressions in NPC cell lines CNE1, CNE1-GL, CNE-2Z and C666-1 were examined by RT-PCR, western blotting and immunoflucesence, respectively. After CNE1, CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor, quercetagetin, the cell viability, colony formation rate and migration ability were analyzed. Results: Pim-1 expression was negative in well-differentiated CNE1 cells, whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells. Interestingly, CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1. Treatment of CNE1-GL and C666-1 cells with quercetagetin significantly decreased the cell viability, colony formation rate and migration ability but not the CNE1 cells. Conclusions: These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration, and targeting Pim-1 may be a potential treatment for anti-Pim-1 expressed NPCs.