KRT8, FAF1 and PTH1R gene polymorphisms are associated with leg weakness traits in pigs
KRT8, FAF1 and PTH1R gene polymorphisms are associated with leg weakness traits in pigs
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DOI：
10.1007/s11033-012-2301-9
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发表时间：
2013-04-01
影响因子：
2.8
通讯作者：
Wimmers, Klaus
中科院分区：
文献类型：
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作者：
Rangkasenee, Noppawan;Murani, Eduard;Wimmers, Klaus
猪骨和关节功能障碍的易感性，概括为腿无力，主要表现为骨软骨病，是养猪生产中的动物福利和经济问题。本研究的目的是确定功能和位置候选基因角蛋白8(KRT8)、Fas相关因子1(FAF1)和甲状旁腺激素I型受体(PTH1R)的多态性，并评估它们与腿部无力性状的关系。因此，在杜洛克×皮特兰F2群体(Dupi；n=310)和商业大白牛群(n=298)的动物中，骨软骨病病变被评分。此外，在杜皮种群中还观察到了骨矿化特征。在基因KRT8(g.8,039G>；A)、FAF1(g.380,914T>；C)和PTH1R(c.1,672C>；T)中发现了SNPs。KRT8与骨密度和骨矿含量显著相关(P<0.05)。FAF1与所有受检关节的OC病变评分相关(P<0.05)。PTH1R对股骨远端关节软骨损伤评分(P=0.01)和尺骨远端骨骺损伤评分(P=0.05)以及所有关节损伤评分总和(OCsum，P=0.04)以及轻、重度病变动物分组(OCcat，P=0.01)均有显著的显性影响。这项研究揭示了KRT8、FAF1和PTH1R与猪的一些腿部无力相关性状存在关联的明确的遗传统计学证据。
The liability to lesions of dysfunctions of bone and joints in pigs, summarized as leg weakness and mostly expressed as osteochondrosis, is an animal welfare and economic issue in pig production. The objective of this study was to identify polymorphisms in the functional and positional candidate genes keratin 8 (KRT8), Fas-associated factor 1 (FAF1) and parathyroid hormone type I receptor (PTH1R) and to evaluate their association with leg weakness traits. Therefore, osteochondrosis lesions were scored in animals of a Duroc x Pietrain F2 population (DuPi; n = 310) and commercial herds of the breed Large White (n = 298). In addition, bone mineralization traits were observed in DuPi population. SNPs were identified in genes KRT8 (g.8,039G > A), FAF1 (g.380,914T > C) and PTH1R (c.1,672C > T). KRT8 showed significant association with bone mineral density and content (P a parts per thousand currency sign 0.05). FAF1 was association with OC lesions score of all joints inspected (P a parts per thousand currency sign 0.05). PTH1R showed significant dominance effects on OC lesion scores of the distal femur articular cartilage (P = 0.01) and epiphysis of the distal ulna (P = 0.05) as well as sums of scores of all joints (OCsum, P = 0.04) and assignment to groups of either severely or gently affected animals (OCcat, P = 0.01). This study reveals clear genetic-statistical evidence for a link of KRT8, FAF1 and PTH1R with some of leg weakness related traits in pigs.