Endothelial Cell Fibrin Gel Angiogenesis Bead Assay.
Endothelial Cell Fibrin Gel Angiogenesis Bead Assay.
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DOI：
10.1007/978-1-0716-2059-5_25
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发表时间：
2022-01-01
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影响因子：
--
通讯作者：
Meakin, Paul J
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文献类型：
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作者：
Clavane, Eva M;Taylor, Hannah A;Meakin, Paul J
纤维蛋白凝胶血管生成珠测定提供了一种受控的体外环境，用于观察内皮血管生成发芽对修改后的变量的反应。将内皮细胞包被在微载体上并包埋在含有必需生长因子的纤维蛋白凝块中。孵育24小时后，使用光学显微镜对内皮芽进行成像。该方法可用于快速、经济地研究基因或化学操作对内皮功能的影响。
The fibrin gel angiogenesis bead assay provides a controlled in vitro setting for observing endothelial angiogenic sprouting in response to modified variables. Endothelial cells are coated onto microcarriers and embedded into a fibrin clot containing necessary growth factors. Following a 24-h incubation, endothelial sprouts are imaged using a light microscope. This method is useful for rapidly and affordably investigating the effects of genetic or chemical manipulation to endothelial function.