Meiotic progression of rat spermatocytes requires mitogen-activated protein kinases of Sertoli cells and close contacts between the germ cells and the Sertoli cells
Meiotic progression of rat spermatocytes requires mitogen-activated protein kinases of Sertoli cells and close contacts between the germ cells and the Sertoli cells
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DOI：
10.1016/j.ydbio.2007.12.019
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发表时间：
2008-03-01
影响因子：
2.7
通讯作者：
Durand, Philippe
中科院分区：
文献类型：
--
作者：
Godet, Murielle;Sabido, Odile;Durand, Philippe
生殖细胞通过减数分裂的进展受到磷酸化事件的调节。我们之前展示了细胞周期蛋白依赖性激酶在与支持细胞（SC）共培养的大鼠精母细胞减数分裂中的关键作用。在本研究中，我们使用相同的培养系统来研究丝裂原激活蛋白激酶（MAPK）在减数分裂进展中的作用。早在接种到 SC 上 3 小时后，就在体内、新鲜分离的 SC 和粗线期精母细胞 (PS) 中检测到磷酸化的 ERK1/2。在用 U0126（ERK 激活激酶 MEK1/2 的抑制剂）处理的共培养物中，两次减数分裂的产量以及高度 MPM-2 标记的粗线期和次级精母细胞 (SII) 的百分比均有所下降。 PS 与 U0126 预孵育导致体外形成的圆形精子细胞数量减少，但不改变 SII 的数量或 PS 或 SII 的 MPM-2 标记。相反，用 U0126 预处理 SC 导致高度 MPM-2 标记的 PS 百分比下降，与 SII 和圆形精子细胞数量减少相关。这些结果表明，精母细胞减数分裂进程依赖于 SC 激活的 MAPK。此外，在支持细胞条件培养基中单独培养的 PS 并未获得高 MPM-2 标记，这表明生殖细胞和 SC 之间对细胞-细胞接触的特殊需要。 (C) 2007 Elsevier Inc. 保留所有权利。
Progression of germ cells through meiosis is regulated by phosphorylation events. We previously showed the key role of cyclin dependent kinases in meiotic divisions of rat spermatocytes co-cultured with Sertoli cells (SC). In the present study, we used the same culture system to address the role of mitogen-activated protein kinases (MAPKs) in meiotic progression. Phosphorylated ERK1/2 were detected in vivo and in freshly isolated SC and in pachytene spermatocytes (PS) as early as 3 h after seeding on SC. The yield of the two meiotic divisions and the percentage of highly MPM-2-labeled pachytene and secondary spermatocytes (SII) were decreased in co-cultures treated with U0126, an inhibitor of the ERK-activating kinases, MEK1/2. Pre-incubation of PS with U0126 resulted in a reduced number of in vitro formed round spermatids without modifying the number of SII or the MPM-2 labeling of PS or SII. Conversely, pre-treatment of SC with U0126 led to a decrease in the percentage of highly MPM-2-labeled PS associated with a decreased number of SII and round spermatids, These results show hat meiotic progression of spermatocytes is dependent on SC-activated MAPKs. In addition, high MPM-2 labeling was not acquired by PS cultured alone in Sertoli cell conditioned media, indicating a specific need for cell-cell contact between germ cells and SC. (C) 2007 Elsevier Inc. All rights reserved.