MicroRNA expression profiles in conventional and micropropagated strawberry (Fragaria x ananassa Duch.) plants
MicroRNA expression profiles in conventional and micropropagated strawberry (Fragaria x ananassa Duch.) plants
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传统和微繁殖草莓 (Fragaria x ananassa Duch.) 植物中的 MicroRNA 表达谱
DOI：
10.1007/s00299-009-0693-3
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发表时间：
2009-06-01
影响因子：
6.2
通讯作者：
Ma, Yue
中科院分区：
文献类型：
--
作者：
Li, He;Zhang, Zhihong;Ma, Yue
MicroRNAs(MiRNAs)是一类在植物生长发育中起关键作用的非编码小RNA。为了检测草莓miRNAs，并发现草莓常规植株和草莓微繁殖植株之间的表达差异，我们利用基因芯片对草莓miRNAs进行了检测和定量。结果表明，在草莓植株中共检测到74个miRNAs，其中4个miRNA基因在草莓常规植株和微繁植株中表现出明显的表达差异，其中2个基因表达上调(miR535和miR390)，2个基因表达下调(miR169a和miR169d)。MiR535、miR390、miR169a和miR169d的草莓常规繁殖株/微繁株比分别为2.6884、2.2673、0.2496和0.3814。对两个上调基因(miR535和miR390)进行定量逆转录聚合酶链式反应，验证了微阵列结果。这是关于miRNAs在常规植物和微繁殖植物中差异表达的首次报道。
MicroRNAs (miRNAs) are a class of small non-coding RNAs which play a critical role in plant growth and development. To detect strawberry miRNAs and discover the expression difference between conventional and micropropagated strawberry plants, we carried out the detection and quantification of strawberry miRNAs by microarray. The main findings were that 74 miRNAs were checked in strawberry plants and four miRNA genes displayed clear expression difference between conventional and micropropagated strawberry plants, including two up-regulated genes (miR535 and miR390) and two down-regulated genes (miR169a and miR169d). The ratios of conventionally propagated strawberry plant/micropropagated strawberry plant for miR535, miR390, miR169a and miR169d were 2.6884, 2.2673, 0.2496 and 0.3814, respectively. Quantitative reverse transcription polymerase chain reaction applied to the two up-regulated genes (miR535 and miR390) validated the microarray result. This is the first report on differential expression of miRNAs in conventional and micropropagated plants.