The MIC1 microneme protein of Toxoplasma gondii contains a duplicated receptor-like domain and binds to host cell surface
The MIC1 microneme protein of Toxoplasma gondii contains a duplicated receptor-like domain and binds to host cell surface
复制标题
DOI：
10.1016/s0166-6851(96)02773-9
复制
发表时间：
1996-12-20
影响因子：
1.5
通讯作者：
Dubremetz, JF
中科院分区：
文献类型：
--
作者：
Fourmaux, MN;Achbarou, A;Dubremetz, JF
编码弓形虫微线体蛋白MIC1的cDNA及相应基因已被克隆并测序，MIC1基因含有3个内含子。该cDNA编码456个氨基酸（aa）序列，具有典型的信号序列，没有其他跨膜结构域。该蛋白含有一个具有半胱氨酸保守性的串联重复结构域，并与疟原虫 sp、微线体蛋白 TRAP-SSP2 具有远距离同源性。来自速殖子裂解物的 MIC1 蛋白和表达该蛋白 N 末端重复结构域的 PMAL 重组体与假定的宿主细胞表面结合，表明 MIC1 可能参与宿主细胞的结合/识别。版权所有 (C) 1996 Elsevier Science B.V.
The cDNA encoding the Toxaplasma gondii microneme protein MIC1 and the corresponding gene have been cloned and sequenced, The MIC1 gene contains three introns. The cDNA encodes a 456 amino acid (aa) sequence, with a typical signal sequence and no other trans-membrane domain. The protein contains a tandemly duplicated domain with conservation of cysteines and presents distant homology with the Plasmodium sp, microneme protein TRAP-SSP2. The MIC1 protein from tachyzoite lysates and a PMAL recombinant expressing the N-terminal duplicated domain of the protein bound to the surface of putative host cells, suggesting a possible involvement of MIC1 in host cell binding/recognition. Copyright (C) 1996 Elsevier Science B.V.