Bovine mononuclear leukocyte subpopulations in peripheral blood and mammary gland secretions during lactation.
Bovine mononuclear leukocyte subpopulations in peripheral blood and mammary gland secretions during lactation.
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DOI：
10.3168/jds.s0022-0302(92)77842-4
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发表时间：
1992-04
影响因子：
3.5
通讯作者：
Y. H. Park;Lawrence K. Fox;M. Hamilton;William C. Davis
中科院分区：
文献类型：
--
作者：
Y. H. Park;Lawrence K. Fox;M. Hamilton;William C. Davis
采用荧光流式细胞术和单克隆抗体分析外周血和乳腺分泌物中白细胞的组成。在哺乳周期的六个时间点从不含 IMI 的荷斯坦奶牛中获取样品。外周血单个核细胞中单核细胞的百分比为15%至31%；最低百分比发生在非哺乳期早期。乳腺分泌物中 T 淋巴细胞的百分比从围产期的 16% 到哺乳后期的 62% 不等。 B 淋巴细胞从哺乳后期的 7% 到围产期的 25% 不等。巨噬细胞从 21% 到 69% 不等；最高百分比发生在围产期。外周血和乳腺分泌物中CD4+:CD8+T淋巴细胞的平均比率分别为1.53和0.85。在整个哺乳周期中，存在于乳腺分泌物中的活化 CD8+ T 淋巴细胞亚群共表达了一种新的活化分子 ACT2。 CD8+ T淋巴细胞比例的增加与CD8+、ACT2+ T淋巴细胞百分比的增加相关。活化的CD8+T淋巴细胞可能在针对病原体的局部免疫反应的调节和表达中发挥重要作用。
Florescence flow cytometry and monoclonal antibodies were used to analyze the composition of leukocytes from peripheral blood and mammary gland secretions. Samples were obtained from Holstein dairy cows, free of IMI, at six time points during the lactation cycle. The percentage of monocytes in peripheral blood mononuclear cells varied from 15 to 31%; the lowest percentage occurred at the early nonlactation period. The percentage of T lymphocytes from mammary gland secretions varied from 16% during the periparturient period to 62% at late lactation. The B lymphocytes varied from 7% at late lactation to 25% during the periparturient period. Macrophages varied from 21 to 69%; the highest percentage occurred during the periparturient period. The mean ratio of CD4+:CD8+ T lymphocytes in the peripheral blood and mammary gland secretions was 1.53 and .85, respectively. A subpopulation of activated CD8+ T lymphocytes present in mammary gland secretions throughout the lactation cycle coexpressed a new activation molecule, ACT2. Increases in the proportion of CD8+ T lymphocytes were associated with an increase in the percentage of CD8+, ACT2+ T lymphocytes. Activated CD8+ T lymphocytes may play an important role in the regulation and expression of the local immune response to pathogens.