A VERSATILE ALGINATE DROPLET GENERATOR APPLICABLE FOR MICROENCAPSULATION OF PANCREATIC-ISLETS
A VERSATILE ALGINATE DROPLET GENERATOR APPLICABLE FOR MICROENCAPSULATION OF PANCREATIC-ISLETS
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DOI：
10.1002/jab.770030407
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发表时间：
1992-12-01
期刊：
影响因子：
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通讯作者：
VANSCHILFAGAARDE, R
中科院分区：
文献类型：
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作者：
WOLTERS, GHJ;FRITSCHY, WM;VANSCHILFAGAARDE, R
通过微囊化对胰岛进行免疫隔离的藻酸盐珠应该小、光滑且球形，以确保在胰岛周围形成坚固的藻酸盐-聚赖氨酸-藻酸盐胶囊，具有最佳的生物相容性以及营养物和激素的扩散。然而，在胰岛周围制备小胶囊很困难。我们新设计的空气喷射液滴发生器允许藻酸盐喷嘴和气套的长度和直径发生变化，从而适应所需的珠子尺寸。海藻酸盐液滴在 100 mM CaCl2 溶液中转化为刚性珠。它们的尺寸取决于夹套的直径、空气流速和喷嘴的外径，而生产率取决于藻酸盐上的压力以及喷嘴的直径和长度。当气流或藻酸盐流量超过一定速率时，液滴就会破碎。这项研究描述了这些变量的相互关系，并定义了它们以可接受的生产率可重复生产用于胰岛微胶囊化的光滑和球形珠子的最佳范围。
Alginate beads for immunoisolation of pancreatic islets by microencapsulation should be small, smooth, and spherical in order to ensure that around the islets a strong alginate-polylysine-alginate capsule will be formed with optimal biocompatibility and diffusion of nutrients and hormones. However, the preparation of small capsules around islets is difficult. Our newly designed air jet droplet generator allows for variations in the length and diameter of the alginate nozzle and the air jacket and is in this way adaptable to a required bead size. Alginate droplets are converted into rigid beads in a 100 mM CaCl2 solution. Their size depends upon the diameter of the jacket, the air flow rate, and the outer diameter of the nozzle, whereas the production rate depends upon the pressure on the alginate, and on the diameter and the length of the nozzle. When the air flow or the alginate flow surpasses a certain rate, the droplets are fragmented. This study describes the mutual relationship of these variables and defines their optimal range for reproducible production of smooth and spherical beads for microencapsulation of islets at an acceptable production rate.