Colocalization of insulin-like growth factor-binding protein with insulin-like growth factor I.
Colocalization of insulin-like growth factor-binding protein with insulin-like growth factor I.
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胰岛素样生长因子结合蛋白与胰岛素样生长因子 I 的共定位。
DOI：
10.1152/ajprenal.1991.261.1.f22
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Venkatachalam,MA
中科院分区：
文献类型：
--
作者：
Kobayashi,S;Clemmons,DR;Venkatachalam,MA
我们报道了胰岛素样生长因子I （IGF-I）和25 kda形式的胰岛素样生长因子结合蛋白（IGF-BP-1）在成年大鼠肾脏中的定位。使用兔抗人igf -1抗体和兔抗人IGF-BP-1抗体定位抗原，该抗体针对从羊水中纯化的人25kda IGF-BP-1。免疫组化技术显示，这两种多肽位于相同细胞类型的相同肾元段。乳头状集管染色最强烈。皮层集管和Henle’s袢髓质厚升肢也有中度染色。在收集管中，抗原存在于主细胞中，而不存在于插层细胞中。在远曲小管、皮质厚升肢和推定为Henle's袢薄肢的结构中，只有轻微的染色。然而，致密黄斑缺乏免疫反应性。IGF-I和IGF-BP-1在同一细胞中的共定位支持了从培养细胞研究中得出的观点，即IGF-I的作用可能被存在于同一位置的igf - bp修饰。
We report the localization of insulin-like growth factor I (IGF-I) and a 25-kDa form of insulin-like growth factor-binding protein (IGF-BP-1) in adult rat kidney. The antigens were localized using a rabbit anti-human IGF-I antibody, and a rabbit anti-human IGF-BP-1 antibody raised against human 25-kDa IGF-BP-1 purified from amniotic fluid. Immunohistochemistry by the avidin-biotin peroxidase conjugate technique showed that both peptides are located in the same nephron segments, in the same cell types. The most intense staining was in papillary collecting ducts. There was moderate staining also in cortical collecting ducts and medullary thick ascending limbs of Henle's loop. In collecting ducts the antigens were shown to be present in principal cells but not in intercalated cells. In distal convoluted tubules, cortical thick ascending limbs, and in structures presumptively identified as thin limbs of Henle's loops there was only modest staining. The macula densa, however, lacked immunoreactivity. Colocalization of IGF-I and IGF-BP-1 in the same cells supports the notion, derived from studies on cultured cells, that the actions of IGF-I may be modified by IGF-BPs that are present in the same location.