Controllable Assembly of Enzymes for Multiplexed Lab-on-a-Chip Bioassays with a Tunable Detection Range
Controllable Assembly of Enzymes for Multiplexed Lab-on-a-Chip Bioassays with a Tunable Detection Range
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用于具有可调检测范围的多重芯片实验室生物测定的酶的可控组装
DOI：
10.1002/anie.201801815
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发表时间：
2018-06-18
影响因子：
16.6
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
--
作者：
Xianyu, Yunlei;Wu, Jing;Jiang, Xingyu
Multiplexed analysis of molecules with different concentrations requires assays with a tunable detection range. A strategy is outlined that uses click chemistry to assemble horseradish peroxidase in a controlled fashion to generate enzyme assemblies as probes for multiplexed bioassays. This controllable assembly of enzymes on detection antibodies allows for lab-on-a-chip immunoassays with a tunable detection range from pgmL(-1) to gmL(-1). Simultaneous, multiplexed bioassays of clinically relevant inflammatory biomarkers in serum are demonstrated in one lab-on-a-chip format, with a limit of detection of 0.47pgmL(-1) for interleukin-6, 2.6 pgmL(-1) for procalcitonin, and 40ngmL(-1) for C-reactive protein. This controlled assembly technique provides a multiplexed platform for simultaneous and quantitative analyses of both low-abundance and high-abundance biomarkers with a broad detection range, which holds great promise as a point-of-care platform for biomedical diagnostics.
