Lateral gene transfer generates accessory genes that accumulate at different rates within a grass lineage
Lateral gene transfer generates accessory genes that accumulate at different rates within a grass lineage
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DOI：
10.1111/nph.19272
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发表时间：
2023-10-04
期刊：
影响因子：
9.4
通讯作者：
Dunning，Luke T.
中科院分区：
文献类型：
--
作者：
Raimondeau，Pauline;Bianconi，Matheus E.;Dunning，Luke T.
横向基因转移（Lateral gene transfer，LGT）是指DNA在没有有性生殖的生物体之间的转移。获得的基因代表了在捐赠者基因组中独立进化的遗传新颖性。系统发育方法表明，LGT是广泛存在于整个禾本科，虽然我们知道很少的潜在dynamic.We确定横向收购的基因在五个新的参考基因组从同一grass属（四Alloteropsis semialata和一Alloteropsis angusta）。使用额外的重测序数据为进一步的40 Allotroposisindividuals，我们把每个基因的收购到一个单基因使用随机字符mapping，然后推断率的收益和losss.We检测168横向收购的基因在五个参考基因组（32-100每个基因组）。指数衰减模型表明，LGT的获取率（6-28每Ma）和随后的损失（11-24%每Ma）显着不同的血统。横向获得基因的丢失率高于纵向遗传基因的丢失率（0.02-0.8%/Ma），这种高周转率造成了种内基因含量的变异，其中绝大多数基因作为辅助基因出现在异特灵属植物基因组中。这种快速的更替产生了持续的变化，最终可以促进当地的适应。
Lateral gene transfer (LGT) is the movement of DNA between organisms without sexual reproduction. The acquired genes represent genetic novelties that have independently evolved in the donor's genome. Phylogenetic methods have shown that LGT is widespread across the entire grass family, although we know little about the underlying dynamics.We identify laterally acquired genes in fivede novoreference genomes from the same grass genus (fourAlloteropsis semialataand oneAlloteropsis angusta). Using additional resequencing data for a further 40Alloteropsisindividuals, we place the acquisition of each gene onto a phylogeny using stochastic character mapping, and then infer rates of gains and losses.We detect 168 laterally acquired genes in the five reference genomes (32–100 per genome). Exponential decay models indicate that the rate of LGT acquisitions (6–28 per Ma) and subsequent losses (11–24% per Ma) varied significantly among lineages. Laterally acquired genes were lost at a higher rate than vertically inherited loci (0.02–0.8% per Ma).This high turnover creates intraspecific gene content variation, with a preponderance of them occurring as accessory genes in theAlloteropsispangenome. This rapid turnover generates standing variation that can ultimately fuel local adaptation.