Exposure to perfluorinated compounds: in vitro study on thyroid cells
Exposure to perfluorinated compounds: in vitro study on thyroid cells
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DOI：
10.1007/s11356-014-3480-9
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发表时间：
2015-02-01
影响因子：
5.8
通讯作者：
Chiovato, Luca
中科院分区：
文献类型：
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作者：
Coperchini, Francesca;Pignatti, Patrizia;Chiovato, Luca
全氟辛酸（PFOA）和全氟辛烷磺酸（PFOS）是广泛使用的全氟化学品。先前的研究在包括甲状腺在内的人体组织中检测到了全氟辛酸和全氟辛烷磺酸。没有关于全氟辛酸和全氟辛烷磺酸对甲状腺细胞的体外影响的研究。我们的研究旨在评估体外暴露于PFOA和PFOS对甲状腺细胞增殖和活力的影响。使用Fisher大鼠甲状腺系-5（FRTL-5）细胞研究了这些目标。在浓度高达10（4）nM的PFOA和PFOS存在下培养的FRTL-5细胞的活力和增殖率没有显示出变化，而在浓度为10（5）nM的任何一种PFCs下，细胞增殖受到显着抑制，主要是由于细胞死亡增加，FRTL-5细胞团与全氟辛烷磺酸孵育72小时后，检测到全氟辛酸和全氟辛烷磺酸，但在对照培养物中未检测到。当FRTL-5与全氟辛烷磺酸一起孵育，然后在PBS中洗涤，并在培养基中不含全氟辛烷磺酸的情况下再培养72小时时，在细胞沉淀物中测得的全氟辛酸和全氟辛烷磺酸浓度不可检测。这表明全氟辛酸和全氟辛烷磺酸通过梯度被动扩散机制进入甲状腺细胞。未来的研究需要评估潜在的毒性效应所造成的长期在体内暴露于更低浓度的全氟化碳。
Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) are widely used perfluorinated chemicals (PFCs). Previous studies detected PFOA and PFOS in human tissues including the thyroid gland. There are no studies on the in vitro effects of PFOA and PFOS on thyroid cells. Our study was aimed at evaluating the effect of the in vitro exposure to PFOA and PFOS on thyroid cell proliferation and viability. These objectives were investigated using Fisher rat thyroid line-5 (FRTL-5) cells. FRTL-5 cells cultured in the presence of PFOA and PFOS at concentrations up to 10(4) nM do not display changes in their viability and proliferation rate, while at a concentration of 10(5) nM of either PFCs, a significant inhibition of cell proliferation, mainly due to increased cell death, was found. PFOA and PFOS were detected in FRTL-5 cell pellets after 72 h of incubation with PFCs but not in control cultures. When FRTL-5 were incubated with PFCs then washed in PBS and re-cultured for 72 h without PFCs in the medium, no detectable concentrations of PFOA and PFOS were measured in the cell pellet. This indicates that PFOA and PFOS enter thyroid cells by a gradient-based passive diffusion mechanism. Future studies are required to evaluate the potential toxic effect resulting from prolonged in vivo exposure to even lower concentrations of PFCs.