Identification of amino acid residues of AcMNPV P143 protein involved in rRNA degradation and restricted viral replication in BM-N cells from the silkworm Bombyx mori.
Identification of amino acid residues of AcMNPV P143 protein involved in rRNA degradation and restricted viral replication in BM-N cells from the silkworm Bombyx mori.
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DOI：
10.1016/j.virol.2015.08.008
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发表时间：
2015-11
期刊：
影响因子：
3.7
通讯作者：
Rina Hamajima;Michihiro Kobayashi;M. Ikeda
中科院分区：
文献类型：
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作者：
Rina Hamajima;Michihiro Kobayashi;M. Ikeda
我们以前证明，rRNA经历快速和广泛的降解后，感染AcMNPV，这是由AcMNPV P143（Ac-P143）蛋白触发的mostriBM-N细胞。在此，我们发现Ac-P143蛋白的六个氨基酸残基，分布在位置514和599之间，参与BM-N细胞中rRNA的降解。这6个残基在AcMNPV、HycuMNPV、SeMNPV和SpltMNPV的P143蛋白中高度保守，它们在感染BM-N细胞后引起rRNA降解，但在Bm-P143蛋白中仅部分保守，它们在BM-N细胞中不引起rRNA降解。我们还证明，Bm-P143蛋白的仅两个选定的残基（N565 S/L578 F）被相应的Ac-P143蛋白残基取代产生能够在BM-N细胞中触发rRNA降解的突变体Bm-P143蛋白。这些结果表明，BmNPV进化了一个独特的P143蛋白，以逃避抗病毒反应，并允许在B中复制。moricells。
We previously demonstrated that rRNA undergoes rapid and extensive degradation inBombyx moriBM-N cells upon infection with AcMNPV, which is triggered by AcMNPV P143 (Ac-P143) protein. Here, we showed that six amino acid residues of Ac-P143 protein, distributing between positions 514 and 599, are involved in rRNA degradation in BM-N cells. The six residues are highly conserved among P143 proteins from AcMNPV, HycuMNPV, SeMNPV and SpltMNPV, which trigger rRNA degradation in BM-N cells upon infection, but are only partially conserved in Bm-P143 protein, which does not induce rRNA degradation in BM-N cells. We also demonstrated that substitution of only two selected residues (N565S/L578F) of Bm-P143 protein with the corresponding Ac-P143 protein residues generates a mutant Bm-P143 protein that is capable of triggering rRNA degradation in BM-N cells. These results indicate that BmNPV evolved a unique P143 protein to evade the antiviral response and allow replication inB. moricells.