Utility of a low-cost 3-D printed microscope for evaluating esophageal biopsies
Utility of a low-cost 3-D printed microscope for evaluating esophageal biopsies
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低成本 3D 打印显微镜用于评估食管活检的实用性
DOI：
10.1016/j.stlm.2024.100145
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发表时间：
2024
影响因子：
--
通讯作者：
Rosen D
中科院分区：
文献类型：
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作者：
Rosen D
在本文中，我们评估了低成本3D打印显微镜在评估食管活检中的实用性。我们对传统显微镜和我们的3D打印显微镜进行了比较分析，利用了一组从接受筛查内窥镜检查的患者中获得的食管活检样本。两位病理学家通过光学显微镜和使用低成本3D打印显微镜获得的数字图像独立检查了30个食管活检组织（观察者1和2）。将载玻片共识诊断与另外2名独立审查数字图像的病理学家的发现进行比较（观察者3和4）。观察员1（k：0.64）和2（k：0.84）的观察员内一致性基本接近完美。所有四名观察者的灵敏度和阴性预测值均为100%，而特异性范围为59%至100%，阳性预测值范围为21%至100%。仅检查数字图像的两名观察员（3和4）的PPV和特异性较低。总的来说，我们的研究结果表明，远程病理学可以使用高灵敏度和特异性，利用我们的3D打印显微镜产生的图片。
In this manuscript we assessed the utility of a low-cost 3D printed microscope to evaluate esophageal biopsies. We conducted a comparative analysis between the traditional microscope and our 3-D printed microscope, utilizing a set of esophageal biopsy samples obtained from patients undergoing screening endoscopy. Two pathologists independently examined 30 esophageal biopsies by light microscopy and digital images obtained using a low-cost 3D printed microscope (Observer 1 and 2). The glass slide consensus diagnosis was compared to the findings of 2 additional pathologist who independently just reviewed the digital images (Observer 3 and 4). The intra-observer agreement was substantial to almost perfect for observer 1 (k:0.64) and 2 (k:0.84). All four observers had 100 % sensitivity and negative predictive value, whereas specificity ranged from 59 % to 100 % and positive predictive value ranged from 21 % to 100 %. The PPV and specificity were lower for the two Observers (3 and 4) who just examined the digital images. Overall, our results suggest that telepathology may be used with high sensitivity and specificity, utilizing the pictures produced by our 3D-printed microscope.