Liquid-chromatographic assay of diazepam and its major metabolites in plasma.
Liquid-chromatographic assay of diazepam and its major metabolites in plasma.
复制标题
血浆中地西泮及其主要代谢物的液相色谱测定。
作者：
J. Mackichan;W. Jusko;P. Duffner;M. Cohen
本文描述了一种同时定量地西泮及其代谢物n -去甲基地西泮和恶西泮的方法，该方法采用反相液相色谱法，紫外检测波长为254 nm。在pH 11.0下用氯仿萃取后，蒸发有机萃取物，剩余物在小体积流动相中重组，进行色谱分析。仅提取250微克/升的血浆就可检测到低至12至20微克/升的浓度。在浓度为50、250和1000微克/升时，各化合物的最大日内变异系数和日变异系数分别为8.0和10.3%。在14种常用的抗惊厥、镇静和精神治疗药物中，只有卡马西平和劳拉西泮可能干扰对恶西泮或n -去甲基地西泮的分析，或两者兼有。单个样品的总色谱时间小于10分钟。说明了该方法在儿童安定倾向研究中的适用性。
We describe a method for simultaneous quantitation of diazepam and its metabolites, N-desmethyldiazepam and oxazepam, by reversed-phase liquid chromatography with ultraviolet detection at 254 nm. After extraction with chloroform at pH 11.0, the organic extract is evaporated, and the residue is reconstituted in a small volume of mobile-phase and chromatographed. Concentrations as low as 12 to 20 microgram/L can be detected after extraction of only 250 microL of plasma. The maximum within-day and day-to-day coefficients of variation for each compound at concentrations of 50, 250, and 1000 microgram/L are 8.0 and 10.3%, respectively. Of 14 commonly used anticonvulsant, sedative, and psychotherapeutic drugs, only carbamazepine and lorazepam may interfere with analysis for oxazepam or N-desmethyldiazepam, or both. Total chromatography time for a single sample is less than 10 min. Applicability of the method to diazepam disposition studies in children is illustrated.