Estrogen receptor in rat liver: translocation to the nucleus in vivo.
Estrogen receptor in rat liver: translocation to the nucleus in vivo.
复制标题
大鼠肝脏中的雌激素受体：体内易位至细胞核。
DOI：
10.1210/endo-102-2-433
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发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
A. Eisenfeld
中科院分区：
文献类型：
--
作者：
R. Aten;M. Weinberger;A. Eisenfeld
成年雌性大鼠体内炔雌醇（EE2）给药（100微克SC）后，肝脏胞质溶胶的雌二醇特异性结合位点（ESBS）在30和60分钟时显著减少。在30分钟时减少到仅用溶剂处理的大鼠中发现的四分之一。与此同时，核ESBS增加。部分纯化的胞质溶胶和致密的蔗糖纯化的细胞核的ESBS测定后，用氚化雌二醇孵育，使用交换试验条件的凝胶过滤。在交换试验孵育过程中升高温度是必要的，以证明EE 2处理大鼠的纯化细胞核中的大多数ESBS，并表明雌激素附着在这些部位。给予5微克EE 2后，胞浆ESBS的减少和核ESBS的增加较小。相比之下，5微克EE2在显著增加子宫核分数中观察到的ESBS方面与100微克EE2一样有效。在全脑纯化核中发现的低水平ESBS在100微克EE 2给药后没有变化。类固醇特异性和蛋白水解酶敏感性的纯化的细胞核ESBS的治疗大鼠是类似的，部分纯化的细胞溶质ESBS的车辆单独治疗的大鼠。这些数据与ESBS是雌激素受体蛋白一致，其在体内雌激素给药后从肝胞质溶胶易位到细胞核。
Following in vivo ethinyl estradiol (EE2) administration (100 microgram sc) to adult female rats, the estradiol-specific binding sites (ESBS) of liver cytosol were markedly reduced at 30 and 60 min. The reduction at 30 min was to one-quarter of that found in rats treated with vehicle alone. Coincident with this reduction, nuclear ESBS were increased. The ESBS of partially purified cytosol and of dense sucrose-purified nuclei were determined by gel filtration after incubations with tritiated estradiol using exchange assay conditions. An elevated temperature during the exchange assay incubations was necessary to demonstrate most of the ESBS in purified nuclei of EE2-treated rats and suggested that estrogens are attached at these sites. Following administration of 5 microgram EE2, the decrease in cytosol ESBS and the increase in nuclear ESBS were smaller. In contrast, 5 microgram EE2 was as effective as 100 microgram EE2 in substantially increasing the ESBS observed in uterine nuclear fractions. The low level of ESBS found in whole brain purified nuclei was unchanged by 100 microgram EE2 administration. The steroid specificity and proteolytic enzyme sensitivity of the purified nuclear ESBS of treated rats were similar to that of the partially purified cytosol ESBS of rats treated with vehicle alone. The data are consistent with the ESBS being estrogen receptor proteins which translocate from the liver cytosol to the nucleus after estrogen administration in vivo.