An improved method for the isolation of free-circulating plasma DNA and cell-free DNA from other body fluids
An improved method for the isolation of free-circulating plasma DNA and cell-free DNA from other body fluids
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DOI：
10.1196/annals.1448.035
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发表时间：
2008-01-01
期刊：
影响因子：
--
通讯作者：
Fleischhacker, Michael
中科院分区：
文献类型：
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作者：
Kirsch, Christoph;Weickmann, Sabine;Fleischhacker, Michael
游离DNA在肿瘤研究、产前诊断、非恶性疾病诊断等领域都有着广泛的应用前景。血浆、血清和其他体液中游离循环DNA的量通常很低，其分离仍然是一个挑战。在这里，我们评估了新的市售NucleoSpin Plasma XS试剂盒用于从血浆、血清和无细胞支气管灌洗液样品中分离无细胞DNA的应用。NucleoSpin色谱柱证明在DNA产量、纯度和小DNA片段回收方面优于QIAamp系统上级。该方法操作简便、快速、易于标准化，对从事CNAPS领域的实验室有一定的参考价值。
Cell-free DNA is of inareasing interest in different fields, such as cancer research, prenatal diagnosis, and the diagnosis of nonmalignant diseases. The quantity of free-circulating DNA in plasma, serum, and other body fluids is usually low and its isolation is still a challenge. Here we evaluate the application of the new commercially available NucleoSpin Plasma XS Kit for the isolation of cell-free DNA from plasma, serum, and cell-free bronchial lavage samples. The NucleoSpin columns proved superior to the QIAamp system in terms of DNA yield, purity, and retrieval of small DNA fragments. The procedure is fast and easy to standardize and might be valuable for laboratories working in the field of CNAPS.