Paclitaxel and water-soluble poly (L-glutamic acid)-paclitaxel, induce direct chromosomal abnormalities and cell death in a murine metastatic melanoma cell line.
Paclitaxel and water-soluble poly (L-glutamic acid)-paclitaxel, induce direct chromosomal abnormalities and cell death in a murine metastatic melanoma cell line.
复制标题
紫杉醇和水溶性聚（L-谷氨酸）紫杉醇可诱导小鼠转移性黑色素瘤细胞系直接染色体异常和细胞死亡。
作者：
A. Multani;C. Li;M. Ozen;M. Yadav;D. Yu;S. Wallace;S. Pathak
本研究旨在证明紫杉醇和水溶性聚（L-谷氨酸）-紫杉醇（PG-TXL）对小鼠黑色素瘤细胞系（K-1735克隆X-21）中染色体形态、端粒缔合和诱导细胞死亡的影响。用不同浓度（0.1微克、1.0微克、4.0微克和8.0微克/毫升）的单独紫杉醇、单独PG或PG-TXL处理鼠黑色素瘤细胞2小时和4小时，并立即收获而不回收。我们发现：（1）端粒联合的中期分裂相频率增加，（2）中期分裂相聚集并扭曲染色体形态，（3）细胞在中期分裂相聚集（有丝分裂停滞），（4）诱导细胞死亡。用PG-TXL处理的细胞比单独用紫杉醇或PG处理的细胞显示出更多的这种异常。我们的初步结果表明，PG-TXL可能是上级的紫杉醇单独诱导细胞毒作用，这些作用可以介导的各种染色体异常的癌细胞。
The purpose of this study was to demonstrate the effects of paclitaxel and water-soluble poly (L-glutamic acid)-paclitaxel (PG-TXL), on chromosome morphology, telomeric associations, and induction of cell death in a murine melanoma cell line (K-1735 clone X-21). Murine melanoma cells were treated with various concentrations (0.1 microgram, 1.0 microgram, 4.0 micrograms, and 8.0 micrograms/ml) of paclitaxel alone, PG alone, or PG-TXL for 2 hr and 4 hr and harvested immediately without recovery. We found that: (1) the frequency of metaphases with telomeric associations increased, (2) metaphases had clumped and distorted chromosome morphology, (3) cells accumulated in metaphase (mitotic arrest), and (4) cell death had been induced. Cells treated with PG-TXL showed more such abnormalities than did cells treated with either paclitaxel or PG alone. Our preliminary results indicate that PG-TXL may be superior to paclitaxel alone in inducing cytotoxic effects, and these effects could be mediated by various chromosomal abnormalities in cancer cells.