A real time affinity biosensor on an insulated polymer using electric impedance spectroscopy in dielectric microchips
A real time affinity biosensor on an insulated polymer using electric impedance spectroscopy in dielectric microchips
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DOI：
10.1039/c4an00212a
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发表时间：
2014-01-01
期刊：
影响因子：
4.2
通讯作者：
Gamby, Jean
中科院分区：
文献类型：
--
作者：
Kechadi, Mohammed;Sotta, Bruno;Gamby, Jean
本文介绍了柔性塑料在微芯片上结合事件实时监测的研究进展。两个平面碳微电极集成到绝缘的聚对苯二甲酸乙二醇酯微芯片中，与微通道中的溶液没有直接电接触。当生物分子在聚合物表面发生相互作用时，通过微电极/PET微通道/溶液构成的界面可以探测到电阻抗的变化。在纳米摩尔浓度的流动微通道中，用相应的兔抗BSA抗体作为分析物，对固定在聚合物微通道表面的BSA进行了分子识别。确定了两种配体之间亲和反应的平衡缔合常数为5 × 10(7) M-1。这种新装置所获得的良好结果使其成为具有竞争力的生物传感器。
This paper presents development of real time monitoring of binding events on flexible plastic in microchips. Two planar carbon microelectrodes are integrated into an insulated polyethylene terephthalate microchip without direct electrical contact with the solution in the microchannel. It has been possible to probe the electric impedance changes through the interface constituted by the microelectrode/PET microchannel/solution when a biomolecular interaction takes place on the polymer surface. This new transduction for biosensing was demonstrated for the molecular recognition of BSA immobilized on the polymer microchannel surface using the corresponding rabbit anti-BSA antibodies as an analyte in the flow microchannel at the nanomolar range concentration. The equilibrium association constant was determined for the affinity reaction between both ligands and was obtained equal to 5 x 10(7) M-1. The promising results obtained with this new device make it a competitive biosensor.