Development of IL-17-mediated Delayed-Type Hypersensitivity Is Not Affected by Down-Regulation of IL-25 Expression
Development of IL-17-mediated Delayed-Type Hypersensitivity Is Not Affected by Down-Regulation of IL-25 Expression
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DOI：
10.2332/allergolint.10-oa-0218
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发表时间：
2010-12-01
影响因子：
6.8
通讯作者：
Nakae, Susumu
中科院分区：
文献类型：
--
作者：
Ishii, Akina;Oboki, Keisuke;Nakae, Susumu
背景资料：IL-25是IL-17家族的一员，诱导Th 2细胞分化和Th 2细胞因子产生，有助于诱导Th 2型免疫应答和疾病，因此它抑制Th 1型和Th 17型免疫应答。为了阐明IL-25在IL-17介导的迟发型超敏反应（DTH）发病机制中的作用，用甲基化BSA（mBSA）致敏IL-25缺陷小鼠，然后用mBSA攻击诱导DTH反应。基于细胞增殖和细胞因子产生评估mBSA特异性T细胞诱导。结果：IL-25在DTH皮损中的表达明显减少，而在正常皮肤中的表达则明显减少。然而，mBSA特异性的Th 1，Th 2和Th 17细胞诱导，和mBSA诱导的DTH反应在IL-25缺陷型和野生型mice.Conclusions：IL-25是不是必不可少的分化的Th 1，Th 2和Th 17细胞在致敏阶段或诱导局部炎症的mBSA诱导的DTH反应的激发阶段。
Background: IL-25, which is a member of the IL-17 family, induces Th2 cell differentiation and Th2 cytokine production, contributing to induction of Th2-type immune responses and diseases, as a result of which it suppresses Th1- and Th17-type immune responses.Methods: To elucidate the role of IL-25 in the pathogenesis of IL-17-mediated delayed-type hypersensitivity (DTH), IL-25-deficient mice were sensitized with methylated BSA (mBSA), and then a DTH reaction was induced by mBSA challenge. mBSA-specific T-cell induction was assessed on the basis of cell proliferation and cytokine production. The DTH reaction was evaluated on the basis of tissue swelling, histology and inflammatory mediator expression.Results: IL-25 expression was markedly reduced in local DTH lesions. However, mBSA-specific Th1, Th2 and Th17 cell induction, and the mBSA-induced DTH reaction were comparable in IL-25-deficient and wild-type mice.Conclusions: IL-25 is not essential for differentiation of Th1, Th2 and Th17 cells in the sensitization phase or induction of local inflammation in the elicitation phase of the mBSA-induced DTH reaction.