INTERACTION OF THE 2 COMPONENTS OF LEUKOCIDIN FROM STAPHYLOCOCCUS-AUREUS WITH HUMAN POLYMORPHONUCLEAR LEUKOCYTE MEMBRANES - SEQUENTIAL BINDING AND SUBSEQUENT ACTIVATION
INTERACTION OF THE 2 COMPONENTS OF LEUKOCIDIN FROM STAPHYLOCOCCUS-AUREUS WITH HUMAN POLYMORPHONUCLEAR LEUKOCYTE MEMBRANES - SEQUENTIAL BINDING AND SUBSEQUENT ACTIVATION
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DOI：
10.1128/iai.62.8.3184-3188.1994
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发表时间：
1994-08-01
影响因子：
3.1
通讯作者：
FINCKBARBANCON, V
中科院分区：
文献类型：
--
作者：
COLIN, DA;MAZURIER, I;FINCKBARBANCON, V
在 1 mM Ca2+ 存在下，研究了来自金黄色葡萄球菌的杀白细胞素的两个组分 S 和 F 与人多形核中性粒细胞膜之间的顺序相互作用。对于 I-125 标记的组分，结果表明仅在 S 组分结合后才发生 F 组分的结合。两种成分的结合动力学常数没有统计学差异（K-d，大约 5 nM；B-m，每个细胞大约 35,000 个分子），并且两个 Hill 系数均为 1。通过氨基己糖苷酶和溶菌酶活性测量确定，增加杀白细胞素浓度的应用引发了颗粒内容物的剂量依赖性分泌。此外，仅当S组分的灌注先于F组分的灌注时，将S和F组分单独灌注到沉积在过滤器上的人多形核中性粒细胞上才诱导颗粒内容物的分泌。因此，我们得出结论，(i) S 成分结合是 F 成分结合和随后多形核中性粒细胞激活的先决条件，并且 (ii) 质膜中存在 S 成分的特异性结合位点。
The sequential interaction between the two components S and F of leukocidin from Staphylococcus aureus and the membrane of human polymorphonuclear neutrophils has been investigated in the presence of 1 mM Ca2+ With I-125-labeled components, it has been shown that binding of the F component occurred only after binding of the S component. The kinetic constants of binding of both components were not statistically different (K-d, approximately 5 nM; B-m, approximately 35,000 molecules per cell), and both Hill coefficients were 1. The application of increasing concentrations of leukocidin provoked a dose-dependent secretion of the granule content, as determined by hexosaminidase and lysozyme activity measurements. Furthermore, the separate perfusion of S and F components on human polymorphonuclear neutrophils deposited on a filter induced secretion of the granules content only when the perfusion of the S component preceded that of the F component. We conclude, therefore, that (i) S-component binding is a prerequisite for F-component binding and for subsequent activation of polymorphonuclear neutrophils and (ii) there is a specific binding site for the S component in the plasma membrane.