Semliki Forest virus and Sindbis virus, but not vaccinia virus, require glycolysis for optimal replication
Semliki Forest virus and Sindbis virus, but not vaccinia virus, require glycolysis for optimal replication
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DOI：
10.1099/jgv.0.000226
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发表时间：
2015-09-01
影响因子：
3.8
通讯作者：
Ulaeto, David
中科院分区：
文献类型：
--
作者：
Findlay, James S.;Ulaeto, David
病毒是专性细胞内病原体，其依赖于细胞的机制来产生复制所需的能量和大分子。感染与改变的代谢谱相关，并且可以改变的一个途径是糖酵解。在这项研究中，我们研究了糖酵解途径是否是甲病毒复制所必需的。用三种不同的糖酵解抑制剂（2-脱氧葡萄糖、氯尼达明和草氨酸盐）预处理Vero细胞导致塞姆利基森林病毒和辛德毕斯病毒复制显著减少（但不消除），但不影响无关病毒牛痘病毒。病毒产量降低与任何显著的细胞毒性效应无关，并且延迟治疗至感染后3 h仍导致显著降低。这表明，通过支持进入后生命周期步骤，糖酵解是甲病毒最佳复制所必需的。
Viruses are obligate intracellular pathogens which rely on the cell's machinery to produce the energy and macromolecules required for replication. Infection is associated with a modified metabolic profile and one pathway which can be modified is glycolysis. In this study, we investigated if the glycolysis pathway is required for alphavirus replication. Pre-treatment of Vero cells with three different glycolysis inhibitors (2-deoxyglucose, lonidamine and oxamate) resulted in a significant reduction (but not abrogation) of Semliki Forest virus and Sindbis virus replication, but not of the unrelated virus, vaccinia virus. Reduced virus yield was not associated with any significant cytotoxic effect and delayed treatment up to 3 h post-infection still resulted in a significant reduction. This suggested that glycolysis is required for optimal replication of alphaviruses by supporting post-entry life cycle steps.