Anticarcinogenic effects of glycoalkaloids from potatoes against human cervical, liver, lymphoma, and stomach cancer cells
Anticarcinogenic effects of glycoalkaloids from potatoes against human cervical, liver, lymphoma, and stomach cancer cells
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DOI：
10.1021/jf050620p
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发表时间：
2005-07-27
影响因子：
6.1
通讯作者：
Kozukue, N
中科院分区：
文献类型：
--
作者：
Friedman, M;Lee, KR;Kozukue, N
设计了用于从出岛马铃薯中分离大量纯α-查康宁和α-龙葵碱以及用于从五个新鲜马铃薯品种（出岛、Jowon、Sumi、Toya和Vora Valley）中提取和分析总配糖生物碱的方法。然后在实验中使用四唑微量培养物（MTT）测定来评价这些化合物，以评估（a）单独分离的纯配糖生物碱、（B）两种配糖生物碱的人工混合物和（c）从五种马铃薯品种中的每一种分离的总配糖生物碱的抗癌作用。所有测试的样品减少了以下人细胞系的数量：宫颈（HeLa）、肝（HepG 2）、淋巴瘤（U937）、胃癌（AGS和KATO 111）细胞和正常肝（Chang）细胞。结果表明：（a）在0.1-10 μ g/mL范围内，配糖生物碱的作用呈浓度依赖性（B）α-茶乌头碱比α-龙葵碱更有活性;（c）某些混合物表现出协同效应，而另一些则产生相加效应;（d）不同的癌细胞对破坏的敏感性不同;和（e）正常肝细胞的破坏通常低于癌肝细胞的破坏。还通过反相显微镜目视观察到细胞群减少。该结果补充了有关食品成分抗癌潜力的相关观察。
Methods were devised for the isolation of large amounts of pure alpha-chaconine and a-solanine from Dejima potatoes and for the extraction and analysis of total glycoalkaloids from five fresh potato varieties (Dejima, Jowon, Sumi, Toya, and Vora Valley). These compounds were then evaluated in experiments using a tetrazolium microculture (MTT) assay to assess the anticarcinogenic effects of (a) the isolated pure glycoalkaloids separately, (b) artificial mixtures of the two glycoalkaloids, and (c) the total glycoalkaloids isolated from each of the five potato varieties. All samples tested reduced the numbers of the following human cell lines: cervical (HeLa), liver (HepG2), lymphoma (U937), stomach (AGS and KATO 111) cancer cells and normal liver (Chang) cells. The results show that (a) the effects of the glycoalkaloids were concentration dependent in the range of 0.1-10 mu g/mL (0.117-11.7 nmol/mL); (b) alpha-chaconine was more active than was alpha-solanine; (c) some mixtures exhibited synergistic effects, whereas other produced additive ones; (d) the different cancer cells varied in their susceptibilities to destruction; and (e) the destruction of normal liver cells was generally lower than that of cancer liver cells. The decreases in cell populations were also observed visually by reversed-phase microscopy. The results complement related observations on the anticarcinogenic potential of food ingredients.