Microtubules: evidence for 13 protofilaments.
Microtubules: evidence for 13 protofilaments.
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DOI：
10.1083/jcb.59.2.267
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发表时间：
1973-11
期刊：
影响因子：
--
通讯作者：
Snyder DH
中科院分区：
文献类型：
--
作者：
Tilney LG;Bryan J;Bush DJ;Fujiwara K;Mooseker MS;Murphy DB;Snyder DH
当在单宁酸存在下将微管固定在戊二醛中并切割薄片时，无需图像增强就可以很容易地观察到微管的亚基结构。分析了7种类型的微管，分别是：日生动物轴突、有丝分裂器、收缩轴突、鸡脑再生微管、鞭毛中心对、鞭毛和基体的A小管。在所有情况下，微管由13个等间距的原丝组成。鞭毛和基体内的B小管似乎由11个亚基组成。描述了B和A以及C和B之间的联系。提出了一种微管模型。
When microtubules are fixed in glutaraldehyde in the presence of tannic acid and thin sections cut, the subunit structure of the microtubule is readily observed without the need of image reinforcement. Seven types of microtubules were analyzed: those in the heliozoan axoneme, the mitotic apparatus, the contractile axostyle, repolymerized microtubules derived from the chick brain, the central pair in flagella, and the A tubules of flagella and the basal body. In all cases microtubules were composed of 13 equally spaced protofilaments. The B tubules in flagella and the basal body appear to be composed of 11 subunits. The connections of the B to the A and the C to the B are described. A model of a microtubule is presented.