T4 bacteriophage as a phage display platform.
T4 bacteriophage as a phage display platform.
复制标题
DOI：
10.1007/s00203-014-0989-8
复制
发表时间：
2014-07
影响因子：
2.8
通讯作者：
Dabrowska, Krystyna
中科院分区：
文献类型：
--
作者：
Gamkrelidze, Mariam;Dabrowska, Krystyna
对T4感染的大肠杆菌分子事件的分析揭示了一些最重要的生物学原理，包括基因及其产物的结构之间的关系，病毒诱导的代谢功能的获得，以及通过顺序基因产物相互作用而不是顺序基因激活来实现复杂结构的形态发生。T4噬菌体和相关菌株被应用于许多基本生物学概念的第一次配方中。这些问题包括对核酸作为遗传物质的明确认识，通过精细结构突变定义基因，重组和功能分析，证明遗传密码是三联体，信使核糖核酸的发现，重组和DNA复制的重要性，光依赖和非光依赖的DNA修复机制，DNA的限制和修饰，原核生物内含子/外显子排列的自我剪接，翻译旁路等。噬菌体T4具有独特的功能，使其成为多组分疫苗平台的良好工具。HOC/SOC融合抗原在体外和体内均可组装在T4衣壳上。T4噬菌体展示结合亲和层析可作为一种新的噬菌体纯化方法。T4噬菌体展示系统也可以作为一种有吸引力的癌症治疗方法。这些数据显示了单个和多个HIV抗原在噬菌体T4衣壳上的有效展示，并为设计新的颗粒HIV或其他尚未被其他载体系统展示的疫苗提供了见解。
Analysis of molecular events in T4-infected Escherichia coli has revealed some of the most important principles of biology, including relationships between structures of genes and their products, virus-induced acquisition of metabolic function, and morphogenesis of complex structures through sequential gene product interaction rather than sequential gene activation. T4 bacteriophages and related strains were applied in the first formulations of many fundamental biological concepts. These include the unambiguous recognition of nucleic acids as the genetic material, the definition of the gene by fine-structure mutation, recombinational and functional analyses, the demonstration that the genetic code is triplet, the discovery of mRNA, the importance of recombination and DNA replications, light-dependent and light-independent DNA repair mechanisms, restriction and modification of DNA, self-splicing of intron/exon arrangement in prokaryotes, translation bypassing and others. Bacteriophage T4 possesses unique features that make it a good tool for a multicomponent vaccine platform. Hoc/Soc-fused antigens can be assembled on the T4 capsid in vitro and in vivo. T4-based phage display combined with affinity chromatography can be applied as a new method for bacteriophage purification. The T4 phage display system can also be used as an attractive approach for cancer therapy. The data show the efficient display of both single and multiple HIV antigens on the phage T4 capsid and offer insights for designing novel particulate HIV or other vaccines that have not been demonstrated by other vector systems.
登录
查看更多内容
影响因子：
3
作者：
BASCHONG, W;BASCHONGPRESCIANOTTO, C;AEBI, U
通讯作者：
AEBI, U
影响因子：
1.6
作者：
Kutter, E;Gachechiladze, K;Mesyanzhinov, V
通讯作者：
Mesyanzhinov, V
DOI：
10.1038/nrg2432
发表时间：
2008-10
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1073/pnas.0400444101
发表时间：
2004-04-20
影响因子：
11.1
作者：
Fokine, A;Chipman, PR;Rossmann, MG
通讯作者：
Rossmann, MG
DOI：
10.1073/pnas.1011218107
发表时间：
2010-11-23
影响因子：
11.1
作者：
Bartual, Sergio G.;Otero, Jose M.;van Raaij, Mark J.
通讯作者：
van Raaij, Mark J.