Characterization of a novel polysaccharide purified from a herb of Cynomorium songaricum Rupr.
Characterization of a novel polysaccharide purified from a herb of Cynomorium songaricum Rupr.
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DOI：
10.1016/j.foodhyd.2015.01.006
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发表时间：
2015-05-01
影响因子：
10.7
通讯作者：
Zhang, Ji
中科院分区：
文献类型：
--
作者：
Wang, Fengxia;Wang, Wei;Zhang, Ji
锁阳（Cynomorium songaricum，Cynomoriaceae）是当地人的一种食物，也是中药中的滋补品。多糖是其活性成分，对其结构进行研究具有重要意义。本研究从锁阳中分离纯化了一个新的多糖组分，命名为CSP-DS 1（分子量为48.1 × 10（4）Da）。通过化学分析和仪器分析，包括酸水解和甲基化分析、红外光谱（IR）、气相色谱-质谱（GC-MS）和核磁共振谱（NMR）（H-1、C-13、H-1-H-1 COSY、HMBC和HMQC）对CSP-DS 1进行了研究。结果表明，CSP-DS 1由三种单糖组成：Man，Glc和Gal，摩尔比约为5.01：89.17：5.82。通过对CSP-DS 1的结构表征和分析，初步确定了CSP-DS 1可能的糖苷键。(C)2015爱思唯尔有限公司版权所有。
Cynomorium songaricum Rupr. (Cynomorium songaricum, Cynomoriaceae) is a food for local people and also a tonic in traditional Chinese medicine. As polysaccharide is an active component in it, it is great significant to study the structure. This research studied a new polysaccharide fraction purified from Cynomorium songaricum, named CSP-DS1 (M-w of 48.1 x 10(4) Da). The CSP-DS1 was investigated by chemical and instrumental analysis, including acid hydrolysis and methylation analysis, infrared spectra (IR), gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR) (H-1, C-13, H-1-H-1 COSY, HMBC and HMQC). The results demonstrate that the CSP-DS1 is comprised of three kinds of monosaccharide: Man, Glc and Gal, in approximately mole ratios of 5.01: 89.17: 5.82. Based on characterization and analysis, its possible glucosidic linkage of the CSP-DS1 is also established. (C) 2015 Elsevier Ltd. All rights reserved.