Rapid changes in ovarian mRNA induced by brief photostimulation in Siberian hamsters (Phodopus sungorus).
Rapid changes in ovarian mRNA induced by brief photostimulation in Siberian hamsters (Phodopus sungorus).
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西伯利亚仓鼠 (Phodopus sungorus) 短暂光刺激引起卵巢 mRNA 的快速变化。
DOI：
10.1002/jez.1953
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发表时间：
2015
期刊：
影响因子：
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通讯作者：
Young,KellyA
中科院分区：
文献类型：
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作者：
Shahed,Asha;McMichael,CarlingF;Young,KellyA
本研究旨在表征关键卵泡生成因子的快速卵巢内mRNA反应，这些因子可能有助于西伯利亚仓鼠在2-10天的光刺激期间恢复卵泡生成。成年仓鼠暴露于短光周期（8L：16 D）14周（SD）。然后将一个子集转移至长光周期（16 L：8 D）2天（PT第2天）、4天（PT第4天）或10天（PT第10天）。使用定量真实的时间PCR测量卵巢内以下mRNA表达：促性腺激素释放激素（GnRH），促卵泡激素β亚单位（FSHβ亚基），促黄体生成素β亚基（LHβ亚基）、FSH和LH受体、雌激素受体α和β（Esr 1和Esr 2）、基质金属蛋白酶（MMP）-2和9、抗苗勒管激素（AMH）、β-胡萝卜素-α亚基，成纤维细胞生长因子-2（FGF-2）和增殖细胞核抗原（PCNA）。与SD相比，PT第4天血浆FSH浓度升高，PT第10天窦卵泡和黄体数量增加。与SD相比，PT第4天的FSHR和FSHR-α mRNA表达也增加，而PT第10天的LHR和增殖标记物PCNA均增加。Esr 1 mRNA在PT第2天增加，与SD相比仍显著增加，而Esr 1 mRNA仅在PT第2天增加，与FGF-2和MMP-2结果相似。光刺激2-10天后，卵巢GnRH、FSHβ-和LHβ-亚基、AMH和MMP-9 mRNA的mRNA表达无差异。卵巢内FSHR和FSH-α mRNA以及窦卵泡/黄体数量的快速增加表明，卵巢对响应于短暂光刺激而释放的FSH做出快速反应。Zool.323A：627-636，2015.© 2015威利期刊公司.
This study sought to characterize the rapid intraovarian mRNA response of key folliculogenic factors that may contribute to the restoration of folliculogenesis during 2–10 days of photostimulation in Siberian hamsters. Adult hamsters were exposed to short photoperiod (8L:16D) for 14 weeks (SD). A subset were then transferred to long photoperiod (16L:8D) for 2 (PT day‐2), 4 (PT day‐4), or 10 days (PT day‐10). Quantitative real‐time PCR was used to measure intraovarian mRNA expression of: gonadotropin releasing hormone (GnRH), follicle stimulating hormone β‐subunit (FSHβ‐subunit), luteinizing hormone β‐subunit (LHβ‐subunit), FSH and LH receptors, estrogen receptors α and β (Esr1 and Esr2), matrix metalloproteinase (MMP)‐2 and ‐9, anti‐Müllerian hormone (AMH), inhibin‐α subunit, fibroblast growth factor‐2 (FGF‐2) and proliferating cell nuclear antigen (PCNA). Compared to SD, plasma FSH concentrations increased on PT day‐4 and the number of antral follicles and corpora lutea increased on PT day‐10. FSHR and inhibin‐α mRNA expression also increased on PT day‐4, whereas LHR and proliferation marker PCNA both increased on PT day‐10 as compared to SD. Esr1 mRNA increased on PT day‐2 and remained significantly increased as compared to SD, whereas Esr1 mRNA increased only on PT day‐2, similar to FGF‐2 and MMP‐2 results. No differences were observed in mRNA expression in ovarian GnRH, FSHβ‐ and LHβ‐subunits, AMH, and MMP‐9 mRNA with 2–10 days of photostimulation. Rapid increases in intraovarian FSHR and inhibin‐α mRNA and antral follicle/corpora lutea numbers suggest that the ovary is primed to react quickly to the FSH released in response to brief periods of photostimulation.J. Exp. Zool. 323A: 627–636, 2015. © 2015 Wiley Periodicals, Inc.