4-SECTOR TANDEM MASS-SPECTROMETRIC ANALYSIS OF COMPLEX-MIXTURES OF PHOSPHATIDYLCHOLINES PRESENT IN A HUMAN-IMMUNODEFICIENCY-VIRUS PREPARATION
4-SECTOR TANDEM MASS-SPECTROMETRIC ANALYSIS OF COMPLEX-MIXTURES OF PHOSPHATIDYLCHOLINES PRESENT IN A HUMAN-IMMUNODEFICIENCY-VIRUS PREPARATION
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DOI：
10.1021/ac00011a010
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发表时间：
1991-06-01
影响因子：
7.4
通讯作者：
HENDERSON, LE
中科院分区：
文献类型：
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作者：
BRYANT, DK;ORLANDO, RC;HENDERSON, LE
通过反相高效液相色谱（HPLC）从HIV - 1/MN制剂中分离出了多种磷脂酰胆碱，并利用快原子轰击质谱（FABMS）、FABMS/MS以及正、负离子模式下的FABMS/MS/MS进行了分析。采用高能碰撞的负离子FABMS/MS来确定酰基的长度、饱和度以及它们在甘油酯基团上的位置。利用正离子模式的FABMS/MS来确定极性头部基团。采用负离子FABMS/MS/MS来确定酰基中双键的位置。我们发现，具有高能碰撞活化的四扇区串联质谱能够对病毒磷脂酰脂质进行相当详细的定性分析以及提供半定量信息。通过测量FABMS中分子离子的相对峰高，对HIV - 1/MN制剂中磷脂酰胆碱含量进行的大致定量分析显示，一系列磷脂酰胆碱与人红细胞中的情况相符，这表明病毒膜中脂质的可能来源是宿主细胞膜。
A number of phosphatidylcholines have been isolated from an HIV-1/MN preparation by reversed-phase high-performance liquid chromatography (HPLC) and analyzed by fast atom bombardment mass spectrometry (FABMS), FABMS/MS, and FABMS/MS/MS in both positive- and negative-ion modes. Negative-ion FABMS/MS with high-energy collisions was used to identify the length of the acyl groups and the degree of saturation, as well as their position on the glyceride group. FABMS/MS in the positive-ion mode was used to identify the polar head group. Negative-ion FABMS/MS/MS was used to locate positions of double bonds in acyl groups. We find that four-sector tandem mass spectrometry with high-energy collisional activation provides qualitative analysis of viral phosphatidyl lipids in considerable detail, as well as semi-quantitative information. Approximate quantitation of the phosphatidylcholine content of the HIV-1/MN preparation by measuring relative peak heights of molecular ions in FABMS reveals an array of phosphatidylcholines consistent with that found in human erythrocytes, indicating the likely source of lipids in the viral membrane to be the host cell membrane.