Proteomic studies in plants.
Proteomic studies in plants.
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DOI：
10.5483/bmbrep.2004.37.1.133
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发表时间：
2004-01
期刊：
影响因子：
--
通讯作者：
Ohkmae K. Park
中科院分区：
文献类型：
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作者：
Ohkmae K. Park
蛋白质组学是在全基因组范围内高通量分析蛋白质的领先技术。随着基因组测序计划的完成和蛋白质分析方法的发展，蛋白质组学已成为功能基因组学的一个重要领域。蛋白质组学的最初目标是大规模鉴定细胞或组织中的所有蛋白质种类。目前的应用正在扩展到分析蛋白质的各种功能方面，如翻译后修饰，蛋白质-蛋白质相互作用，活性和结构。蛋白质组学在动物、酵母和大肠杆菌中的研究已经相当深入，而植物蛋白质组学的研究还处于起步阶段。植物蛋白质组学的研究主要集中在亚细胞蛋白质组和蛋白质复合体（如质膜、叶绿体、线粒体和细胞核中的蛋白质）。这里将介绍几种植物蛋白质组学研究，然后是最近使用多维蛋白质鉴定技术（MudPIT）的工作。
Proteomics is a leading technology for the high-throughput analysis of proteins on a genome-wide scale. With the completion of genome sequencing projects and the development of analytical methods for protein characterization, proteomics has become a major field of functional genomics. The initial objective of proteomics was the large-scale identification of all protein species in a cell or tissue. The applications are currently being extended to analyze various functional aspects of proteins such as post-translational modifications, protein-protein interactions, activities and structures. Whereas the proteomics research is quite advanced in animals and yeast as well as Escherichia coli, plant proteomics is only at the initial phase. Major studies of plant proteomics have been reported on subcellular proteomes and protein complexes (e.g. proteins in the plasma membranes, chloroplasts, mitochondria and nuclei). Here several plant proteomics studies will be presented, followed by a recent work using multidimensional protein identification technology (MudPIT).