INTERACTIONS AMONG CLONAL SUB-POPULATIONS AFFECT STABILITY OF THE METASTATIC PHENOTYPE IN POLYCLONAL POPULATIONS OF B-16 MELANOMA-CELLS
INTERACTIONS AMONG CLONAL SUB-POPULATIONS AFFECT STABILITY OF THE METASTATIC PHENOTYPE IN POLYCLONAL POPULATIONS OF B-16 MELANOMA-CELLS
复制标题
DOI：
10.1073/pnas.78.10.6226
复制
发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
FIDLER, IJ
中科院分区：
文献类型：
--
作者：
POSTE, G;DOLL, J;FIDLER, IJ
从小鼠B16黑色素瘤细胞系（B16-F1和F10）分离的克隆的转移特性的分析显示广泛的细胞异质性和具有广泛不同的转移能力的亚群的存在。这种转移异质性模式在体外和体内连续传代期间得以维持。甚至从这些异源亲本系分离的单个克隆的短连续传代也导致具有不同转移特性的变体亚克隆的快速出现。如果几个克隆混合共培养，这种不稳定性就不会表达。显然，在多克隆种群中，不同的克隆亚群以某种方式相互作用，以稳定它们在种群中的相对比例。通过选择性杀死多克隆群体中的大多数克隆来限制克隆多样性，消除了稳定性限制，并刺激新亚群的快速出现，以产生包含一组新的表型多样性亚群的异源群体，然后达到稳定的比例，直到遇到下一个选择压力。
Analysis of the metastatic properties of clones isolated from mouse B16 melanoma cell lines (B16-F1 and F10) shows extensive cellular heterogeneity and the presence of subpopulations that have widely differing metastatic abilities. This pattern of metastatic heterogeneity is maintained during serial passage in vitro and in vivo. Even a short serial passage of individual clones isolated from these heterogenous parent lines results in rapid emergence of variant subclones that have different metastatic properties. If several clones are mixed and cocultivated this instability is not expressed. Apparently, in polyclonal populations the various clonal subpopulations somehow interact with one another to stabilize their relative proportions within the population. Restriction of clonal diversity by selective killing of the majority of clones in a polyclonal population eliminates the stabilizing restraints and stimulates rapid emergence of new subpopulations to create heterogenous populations containing a new panel of phenotypically diverse subpopulations that then reach stable proportions until the next selection pressure is encountered.