The different molar absorptivities of the secondary structure types in the amide I region: An attenuated total reflection infrared study on globular proteins
The different molar absorptivities of the secondary structure types in the amide I region: An attenuated total reflection infrared study on globular proteins
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DOI：
10.1006/abio.1996.0434
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发表时间：
1996-11-01
影响因子：
2.9
通讯作者：
Ruysschaert, JM
中科院分区：
文献类型：
--
作者：
deJongh, HHJ;Goormaghtigh, E;Ruysschaert, JM
在红外蛋白质光谱的酰胺I区域的各种二级结构的摩尔吸光系数的差异将有很大的影响，迄今为止发表的数据的解释蛋白质薄膜研究的衰减全反射红外光谱，在这项工作中，代表性的酰胺I吸光系数的值获得15个不同的膜的球状蛋白质从水溶液中蔓延。所观察到的强度的膜厚度的变化和水合水，大气物质，和侧链的贡献进行了校正，这些吸收系数，连同报告的二级结构的蛋白质研究，用于推导出的摩尔吸光系数的个别二级结构类型。发现β-链的摩尔吸光系数比α-螺旋的摩尔吸光系数大1.4-1.6倍，而α-螺旋的摩尔吸光系数又比β-转角或无规卷曲结构的摩尔吸光系数大1.3-2.1倍。目前在文献中使用的光谱分析，我们的研究结果的影响进行了讨论。(C)出版社：Academic Press，Inc.
Differences in molar absorptivity of the various secondary structures in the amide I region of infrared protein spectra would have a great impact on the interpretation of the data published thus far on protein films studied by attenuated total reflection infrared spectroscopy, In this work, representative values for amide I absorptivities are obtained for 15 different films of globular proteins spread from H2O solutions. The observed intensities are corrected for variations in film thickness and for contributions of hydration water, atmospheric mater, and side chains, These absorptivities, together with the reported secondary structure of the proteins investigated, are used to deduce the molar absorptivities of the individual secondary structure types. It is found that the molar absorptivity of beta-strands is 1.4-1.6 times larger than that of alpha-helices, which in turn is 1.3-2.1 times larger than those found for beta-turns or random coiled structures. The implications of our findings for spectral analysis currently used in literature are discussed. (C) 1996 Academic Press, Inc.