Echocardiographic Approaches and Protocols for Comprehensive Phenotypic Characterization of Valvular Heart Disease in Mice
Echocardiographic Approaches and Protocols for Comprehensive Phenotypic Characterization of Valvular Heart Disease in Mice
复制标题
DOI：
10.3791/54110
复制
发表时间：
2017-02-01
影响因子：
1.2
通讯作者：
Miller, Jordan D.
中科院分区：
文献类型：
--
作者：
Casaclang-Verzosa, Grace;Enriquez-Sarano, Maurice;Miller, Jordan D.
本手稿和随附视频的目的是概述用于啮齿动物心脏瓣膜功能成像的方法和途径，并详细描述适当的麻醉方法、使用的超声心动图窗口、图像采集的成像平面和探头方向、数据分析方法以及用于评价心脏和瓣膜功能的新兴技术的局限性。重要的是，我们还强调了心脏和心脏瓣膜成像研究的几个未来领域，这些领域可能被用来深入了解临床前动物模型中瓣膜疾病的发病机制。我们建议使用系统的方法来评估小鼠的心脏和心脏瓣膜功能，可以产生更可靠和可重复的数据，并有助于发现遗传改变和/或生理应激小鼠中以前未被充分认识的表型。
The aim of this manuscript and accompanying video is to provide an overview of the methods and approaches used for imaging heart valve function in rodents, with detailed descriptions of the appropriate methods for anesthesia, the echocardiographic windows used, the imaging planes and probe orientations for image acquisition, the methods for data analysis, and the limitations of emerging technologies for the evaluation of cardiac and valvular function. Importantly, we also highlight several future areas of research in cardiac and heart valve imaging that may be leveraged to gain insights into the pathogenesis of valve disease in preclinical animal models. We propose that using a systematic approach to evaluating cardiac and heart valve function in mice can result in more robust and reproducible data, as well as facilitate the discovery of previously underappreciated phenotypes in genetically-altered and/or physiologically-stressed mice.