Endurance training induces depot-specific changes in IL-10/TNF-α ratio in rat adipose tissue
Endurance training induces depot-specific changes in IL-10/TNF-α ratio in rat adipose tissue
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DOI：
10.1016/j.cyto.2008.10.018
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发表时间：
2009-02-01
期刊：
影响因子：
3.8
通讯作者：
Seelaender, M.
中科院分区：
文献类型：
--
作者：
Lira, F. S.;Rosa, J. C.;Seelaender, M.
白色脂肪组织（WAT）是促炎细胞因子和抗炎细胞因子的来源，最近，它被认为是白细胞介素10（IL-10）的重要来源。已知急性体育锻炼诱导抗炎细胞因子谱，然而，慢性体育锻炼对WAT产生IL-10的影响从未被研究过。我们评估了进行耐力训练的大鼠WAT中IL-10和TNF-α的浓度。将动物随机分配到久坐对照组（S，n = 7）或耐力训练组（T，n = 8）。训练大鼠在跑步机上跑步5天/周，持续8周（55- 65%VO2max.检测IL-10和TNF-α蛋白和mRNA表达，以及PPAR-gamma基因表达，免疫细胞化学检测单核吞噬细胞。与S相比，观察到T中的绝对腹膜后脂肪组织（RPAT）重量减少（44%; p < 0.01）。IL-10浓度增加（1.5倍，p < 0.05），其程度高于TNF-α（66%）。P < 0.05），而在RPAT中没有观察到与训练相关的变化。在MEAT中，与S相比，T中的IL-10/TNF-α比率增加（30%; p < 0.05）。在相同的脂肪库中，与S相比，T中的PPAR-gamma基因表达增加（1.1倍; p < 0.01）。总之，运动训练诱导肠系膜贮库中IL-10表达增加，导致IL-10/TNF-α比率改变。我们还得出结论，WAT提出了一个仓库特定的耐力训练方面的研究。(C)2008爱思唯尔有限公司保留所有权利。
White adipose tissue (WAT) is the source of pro- and anti-inflammatory cytokines and recently, it has been recognized as an important source of interleukin 10 (IL-10). Acute physical exercise is known to induce an anti-inflammatory cytokine profile, however, the effect of chronic physical exercise on the production of IL-10 by WAT has never been examined. We assessed IL-10 and TNF-alpha concentration in WAT of rats engaged in endurance training. Animals were randomly assigned to either a sedentary control group (S, n = 7) or an endurance trained group (T, n = 8). Trained rats ran on a treadmill 5 days/wk for 8 wk (55-65% VO2max. Detection of IL-10 and TNF-alpha protein and mRNA expression, as well as the gene expression of PPAR-gamma, and immunocytochemistry to detect mononuclear phagocytes were carried out. A reduction in absolute retroperitoneal adipose tissue (RPAT) weight in T (44%; p < 0.01), when compared with S was observed. IL-10 concentration was increased (1.5-fold, p < 0.05), to a higher extent than that of TNF-alpha (66%. p < 0.05) in the mesenteric adipose tissue (MEAT) of the trained group, while no change related to training was observed in RPAT. In MEAT, IL-10/TNF-alpha ratio was increased in T, when compared with S (30%; p < 0.05). PPAR-gamma gene expression was increased in T (1.1-fold; p < 0.01), when compared with S in the same adipose depot. No monocyte infiltration was found. In conclusion, exercise training induced increased IL-10 expression in the mesenteric depot, resulting in a modified IL-10/TNF-alpha ratio. We also conclude that WAT presents a depot-specific response to endurance training regarding the studied aspects. (C) 2008 Elsevier Ltd. All rights reserved.