Antioxidative and prooxidative effects of coumarin derivatives on free radical initiated and photosensitized peroxidation of human low-density lipoprotein
Antioxidative and prooxidative effects of coumarin derivatives on free radical initiated and photosensitized peroxidation of human low-density lipoprotein
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DOI：
10.1016/s0009-3084(99)00101-2
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发表时间：
1999-12-01
影响因子：
3.4
通讯作者：
Liu, ZL
中科院分区：
文献类型：
--
作者：
Liu, ZQ;Yu, W;Liu, ZL
研究了4-甲基香豆素（MC）、7-羟基-4-甲基香豆素（HMC）和7,8-二羟基-4-甲基香豆素（DHMC）在人低密度脂蛋白（LDL）过氧化过程中的抗氧化活性和/或促氧化活性。过氧化反应是由水溶性引发剂2,2′-偶氮双胍（2-氨基氨基丙烷盐酸盐）（AAPH）热引发的，或由三重态敏化剂二苯甲酮（BP）或其水溶性类似物3,3′-二硫苯并苯甲酸二钠（DSBP）光化学引发的。反应动力学是通过氧气的摄取和天然LDL中cl-生育酚（TOH）的消耗来监测的。动力学分析表明，DHMC对aaph引发的和BP-和dsbp -光敏的过氧化反应都是良好的抗氧化剂；HMC是aaph引发和dsbp光敏过氧化反应的促氧化剂，但对bp敏化过氧化反应是抗氧化剂；MC在所有这些起始条件下都是促氧化剂。香豆素衍生物的抗氧化作用可能包括捕获起始自由基，捕获传播的脂质过氧化自由基，回收α -生育酚和/或使激发的光敏剂失活。1999爱思唯尔科学爱尔兰有限公司版权所有。
The antioxidative and/or prooxidative activity of 4-methylcoumanrin (MC), 7-hydroxy-4-methylcoumarin (HMC) and 7,8-dihydroxy-4-methylcoumarin (DHMC), respectively, in the peroxidation of human low-density lipoprotein (LDL) has been studied. The peroxidation was initiated either thermally by water-soluble initiator 2,2'-azobis(2-amidinopropane hydrochloride) (AAPH), or photochemically by a triplet sensitizer benzophenone (BP) or its water-soluble analogue disodium 3,3'-disulfobenzophenonate (DSBP). The reaction kinetics were monitored by the uptake of oxygen and the depletion of cl-tocopherol (TOH) present in the native LDL. Kinetic analysis of the peroxidation process demonstrated that DHMC is a good antioxidant for both the AAPH-initiated and BP- and DSBP-photosensitized peroxidation; HMC is a prooxidant for the AAPH-initiated and DSBP-photosensitized peroxidation, but an antioxidant for the BP-sensitized peroxidation; MC is a prooxidant in all of these initiation conditions. The antioxidative action of the coumarin derivatives may include trapping the initiating radicals, trapping the propagating lipid peroxyl radicals, recycling alpha-tocopherol and/or deactivating the excited photosensitizer. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.