LUTROPIN-CHORIOGONADOTROPIN RECEPTOR - AN UNUSUAL MEMBER OF THE G-PROTEIN COUPLED RECEPTOR FAMILY
LUTROPIN-CHORIOGONADOTROPIN RECEPTOR - AN UNUSUAL MEMBER OF THE G-PROTEIN COUPLED RECEPTOR FAMILY
复制标题
DOI：
10.1126/science.2502842
复制
发表时间：
1989-08-04
期刊：
影响因子：
56.9
通讯作者：
SEEBURG, PH
中科院分区：
文献类型：
--
作者：
MCFARLAND, KC;SPRENGEL, R;SEEBURG, PH
利用基于纯化受体蛋白的肽序列的寡核苷酸引物，通过聚合酶链反应产生的DNA探针，分离大鼠黄体促黄体激素-绒毛膜促性腺激素受体（LH-CG-R）的互补DNA（cDNA）。如从cDNA序列预测的，LH-CG-R由26个残基的信号肽、显示富含亮氨酸的糖蛋白（LRG）家族成员的内部重复结构特征的341个残基的胞外结构域和含有7个跨膜区段的333个残基的区域组成。该跨膜区域显示与G蛋白偶联受体家族的所有成员的序列相似性。因此，LH-CG-R基因可能是通过LRG和G蛋白偶联受体基因的重组而进化的。表达LH-CG-R cDNA的细胞以高亲和力结合人绒毛膜促性腺激素，并在暴露于激素时显示环磷酸腺苷增加。如RNA印迹分析和原位杂交所示，4.4-脱氢酶同源信使RNA显著定位于大鼠卵巢中。
A complementary DNA (cDNA) for the rat luteal lutropin-choriogonadotropin receptor (LH-CG-R) was isolated with the use of a DNA probe generated in a polymerase chain reaction with oligonucleotide primers based on peptide sequences of purified receptor protein. As would be predicted from the cDNA sequence, the LH-CG-R consists of a 26-residue signal peptide, a 341-residue extracellular domain displaying an internal repeat structure characteristic of members of the leucine-rich glycoprotein (LRG) family, and a 333-residue region containing seven transmembrane segments. This membrane-spanning region displays sequence similarity with all members of the G protein-coupled receptor family. Hence, the LH-CG-R gene may have evolved by recombination of LRG and G protein-coupled receptor genes. Cells engineered to express LH-CG-R cDNA bind human choriogonadotropin with high affinity and show an increase in cyclic adenosine monophosphate when exposed to hormone. As revealed by RNA blot analysis and in situ hybridization, the 4.4-kilobase cognate messenger RNA is prominently localized in the rat ovary.