Effects of periodontal therapy on GCF cytokines in generalized aggressive periodontitis subjects.
Effects of periodontal therapy on GCF cytokines in generalized aggressive periodontitis subjects.
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DOI：
10.1111/j.1600-051x.2011.01817.x
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发表时间：
2012-03
影响因子：
6.7
通讯作者：
Teles RP
中科院分区：
文献类型：
--
作者：
de Lima Oliveira AP;de Faveri M;Gursky LC;Mestnik MJ;Feres M;Haffajee AD;Socransky SS;Teles RP
目的：检测全身性侵袭性牙周炎(GAgP)牙周治疗前后龈沟液(GCF)细胞因子水平的变化。对25名牙周健康受试者和24名GAgP受试者的牙周临床参数进行了测量，并从多达14个部位/受试者采集了牙周沟液(GCF)样本。采用多重微珠免疫分析法检测GM-CSF、干扰素-γ、IL-10、IL-1β、IL-2、IL-6和肿瘤坏死因子-α。侵袭性牙周炎患者随机分为两组，一组为单纯根面刮治(SRP)，另一组为SRP+全身阿莫西林(500 Mg)和甲硝唑(400 Mg)，每日3次，疗程14天。治疗6个月后复查临床参数和牙周液细胞因子。使用Wilcoxon检验分析随时间的差异，并使用Mann-Whitney检验分析组间差异。治疗后GCFGM-CSF、IL-1β及IL-1β/IL-10比值显著降低，而GCFIL-6显著升高。GCF细胞因子的平均变化在不同组之间没有显著差异。牙周治疗通过降低IL-1β和升高IL-10水平来改善牙周病的细胞因子谱。治疗后GCFGM-CSF水平的降低提示该细胞因子参与了GAgP的发病机制。不同治疗方法对牙周膜细胞因子的影响无差异。
To examine changes in levels of gingival crevicular fluid (GCF) cytokines, after periodontal therapy of generalized aggressive periodontitis (GAgP). Twenty five periodontally healthy and 24 GAgP subjects had periodontal clinical parameters measured and gingival crevicular fluid (GCF) samples collected from up to 14 sites/subject. GCF samples were analyzed using multiplex bead immunoassay for: GM-CSF, IFN-γ, IL-10, IL-1β, IL-2, IL-6 and TNF-α. Aggressive periodontitis subjects were randomly assigned to either scaling and root planing (SRP) alone or SRP plus systemic amoxicillin (500 mg) and metronidazole (400 mg) 3 times a day for 14 days. Clinical parameters and GCF cytokines were re-measured 6 months after treatment. Differences over time were analyzed using the Wilcoxon test and between groups using the Mann-Whitney test. Significant reductions in GCF GM-CSF, IL-1β and the ratio IL-1β/IL-10 and increases in GCF IL-6 were detected after therapy. The mean change in GCF cytokines did not differ significantly between groups. Periodontal therapy improved GCF cytokine profiles by lowering IL-1β and increasing IL-10 levels. The reduction in GCF GM-CSF after therapy implicates this cytokine in the pathogenesis of GAgP. There was no difference between therapies in changes of GCF cytokines.