Toward a glucose biosensor based on surface-enhanced Raman scattering
Toward a glucose biosensor based on surface-enhanced Raman scattering
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DOI：
10.1021/ja028255v
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发表时间：
2003-01-15
影响因子：
15
通讯作者：
Van Duyne, RP
中科院分区：
文献类型：
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作者：
Shafer-Peltier, KE;Haynes, CL;Van Duyne, RP
这项工作是利用表面增强拉曼光谱(SERS)构建葡萄糖生物传感器的第一步。在历史上，葡萄糖一直很难用SERS检测，因为它有一个很小的法向拉曼截面，在裸露的银表面上吸附很弱或根本不吸附。在这篇论文中，我们首次系统地研究了用SERS直接检测葡萄糖。葡萄糖被分配到吸附在纳米球(AgFON)表面的银膜上的烷硫醇单分子膜上，从而预先聚集在0-4 nm厚的电磁场增强区域内。本文介绍的实验利用留一偏最小二乘(LOO-PILS)分析来验证在较大(0-250 mm)和临床相关(0-25 mm)浓度范围内的定量血糖检测。在临床研究中，预测的均方根误差(RMSEP)为1.8 mm(33.1 mg/dL)，接近医学应用的要求(11 mm，18 mg/dL)。未来的研究将朝着真正的活体、实时、微创传感方向发展。
This work presents the first step toward a glucose biosensor using surface-enhanced Raman spectroscopy (SERS). Historically, glucose has been extremely difficult to detect by SERS because it has a small normal Raman cross section and adsorbs weakly or not at all to bare silver surfaces. In this paper, we report the first systematic study of the direct detection of glucose using SERS. Glucose is partitioned into an alkanethiol monolayer adsorbed on a silver film over nanosphere (AgFON) surface and thereby, it is preconcentrated within the 0-4 nm thick zone of electromagnetic field enhancement. The experiments presented herein utilize leave-one-out partial least-squares (LOO-PILS) analysis to demonstrate quantitative glucose detection both over a large (0-250 mM) and clinically relevant (0-25 mM) concentration range. The root-mean-squared error of prediction (RMSEP) of 1.8 mM (33.1 mg/dL) in the clinical study is near that desired for medical applications (11 mM, 18 mg/dL). Future studies will advance toward true in vivo, real time, minimally invasive sensing.