Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.
Microfabricated electrochemical cell-based biosensors for analysis of living cells in vitro.
复制标题
基于电化学细胞的微生物生物传感器，用于分析活细胞体外。
DOI：
10.3390/bios2020127
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发表时间：
2012-04-25
期刊：
影响因子：
--
通讯作者：
Wang P
中科院分区：
文献类型：
--
作者：
Wang J;Wu C;Hu N;Zhou J;Du L;Wang P
细胞生化参数可用于揭示各种细胞的生理和功能信息。由于电化学检测方法具有高精度和非侵入性，已被用于基于细胞的研究。当与改进的生物传感器设计和先进的测量系统相结合时，活细胞体外在线生化分析已应用于生物机制研究、药物筛选甚至环境监测。近几十年来，随着微加工技术的发展，新型小型化电化学生物传感器不断涌现。本综述旨在概述基于微制造电化学细胞的生物传感器，例如微电极阵列（MEA）、电池-基底阻抗传感（ECIS）技术和光可寻址电位传感器（LAPS）。详细介绍了其工作原理、测量系统以及在电池监测中的应用。在生物医学提出的高通量和多参数检测需求的驱动下，还介绍了基于电化学细胞的生物传感器的发展趋势，包括新开发的集成生物传感器以及纳米技术和微流控技术的应用。
Cellular biochemical parameters can be used to reveal the physiological and functional information of various cells. Due to demonstrated high accuracy and non-invasiveness, electrochemical detection methods have been used for cell-based investigation. When combined with improved biosensor design and advanced measurement systems, the on-line biochemical analysis of living cells in vitro has been applied for biological mechanism study, drug screening and even environmental monitoring. In recent decades, new types of miniaturized electrochemical biosensor are emerging with the development of microfabrication technology. This review aims to give an overview of the microfabricated electrochemical cell-based biosensors, such as microelectrode arrays (MEA), the electric cell-substrate impedance sensing (ECIS) technique, and the light addressable potentiometric sensor (LAPS). The details in their working principles, measurement systems, and applications in cell monitoring are covered. Driven by the need for high throughput and multi-parameter detection proposed by biomedicine, the development trends of electrochemical cell-based biosensors are also introduced, including newly developed integrated biosensors, and the application of nanotechnology and microfluidic technology.