Phytophthora sojae zoospores differ in chemotaxis to the root and root exudates of host soybean and nonhost common bean
Phytophthora sojae zoospores differ in chemotaxis to the root and root exudates of host soybean and nonhost common bean
复制标题
大豆疫霉游动孢子对寄主大豆和非寄主菜豆的根部和根部分泌物的趋化性不同
DOI：
10.1007/s10327-019-00839-9
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发表时间：
2019-02
影响因子：
1.2
通讯作者：
Wen Jingzhi
中科院分区：
文献类型：
--
作者：
Zhang Zhuoqun;Xu Ying;Song Guangmei;Gao Xinying;Zhao Yuqi;Jia Mengzhen;Chen Yufei;Suo Bing;Chen Qiuming;Wu Di;Wu Wenxu;Wen Jingzhi
大豆疫霉根腐病和茎腐病是许多国家的一种破坏性病害，由土传病原体大豆疫霉引起。土传病原体在入侵前与植物根部之间的相互作用是揭示宿主和非宿主对土传病原体的抗性机制的一个焦点。在本研究中，大豆品种。 Sloan（易感）、Williams 82（抗性）和非寄主普通豆 cv.采用一点红测定根部、根部分泌物以及根部分泌物中的异黄酮、氨基酸、糖和柠檬酸对大豆疫病菌游动孢子侵染前行为（趋向、包囊和胞囊萌发）的影响。两种寄主大豆的根伸长区比非寄主大豆的根伸长区吸引了显着更多的游动孢子，而感病品种的根伸长区比抗性品种吸引了显着更多的游动孢子。同样，寄主大豆根部分泌物吸引游动孢子并促进游动孢子包囊和孢囊萌发，但非寄主大豆根部分泌物对游动孢子没有影响。因此，酱油霉显然根据根部分泌物选择其宿主。在根部分泌物中检测到的 26 种成分中，感病大豆、抗性大豆和非寄主菜豆的根部分泌物分别含有 12 种、17 种和 25 种成分。宿主与非宿主之间的差异主要在于氨基酸的类型和浓度。大多数成分是大豆假单胞菌游动孢子的重要化学引诱剂。然而，当这些引诱剂以其在根系分泌物中的相对浓度混合时，与各成分之和相比，化学引诱力显着降低，且由强到弱依次减少的是非寄主蚕豆、抗性大豆和感病大豆，这表明根系分泌物中这些成分之间存在相互作用，且相互作用的强度与各成分的类型和浓度有关。这种现象是因为某些成分可能共享相同的受体，从而造成阻塞效应。这一结果也可能是菜豆是大豆疫病菌非寄主的一个原因。此外，两种非寄主品种根部分泌物中柠檬酸的相对局部浓度。一点红和抗性品种。 Williams 82 对大豆疫霉的游动孢子具有排斥性。 用两种浓度的柠檬酸对斯隆进行修正，特别是降低了游动孢子的化学吸引力，表明根分泌物中的柠檬酸在宿主和非宿主对大豆疫病菌的抗性中也发挥着重要作用。
Phytophthora root and stem rot of soybean is a destructive disease in many countries caused by the soil-borne pathogen Phytophthora sojae. The interaction between soil-borne pathogens and plant roots before invasion is a focus of interest for revealing host and nonhost resistance mechanisms to soil-borne pathogens. In the present study, soybean cvs. Sloan (susceptible), Williams 82 (resistant) and nonhost common bean cv. Yidianhong were used to determine the effect of roots, root exudates, and isoflavones, amino acids, sugars, and citric acid from the root exudates on the pre-infection behavior (taxis, encystment and cyst germination) of zoospores of P. sojae. The elongation zone of roots of both host soybeans attracted significantly more zoospores than did that of the nonhost bean, and that of the susceptible cultivar attracted significantly more zoospores than did that of the resistant cultivar. Similarly, the host soybean root exudates attracted zoospores and promoted zoospore encystment and cyst germination, but nonhost root exudates had no effect on zoospores. Thus, P. sojae apparently selects its host depending on the root exudates. Of the 26 total components detected from root exudates, the exudates from susceptible and resistant soybeans and nonhost common bean had 12, 17, and 25 components respectively. The differences between the host and nonhost were mainly in the type and concentration of amino acids. Most components were significant chemoattractants of P. sojae zoospores. However, when these attractants were mixed at their relative concentrations in the root exudates, the chemoattraction was significantly reduced compared to that of the sum of each components, and the reduction from strongest to weakest was nonhost common bean, resistant and susceptible soybeans, indicating that these components in the root exudates interact with each other, and the intensity of the interaction relates to the type and concentration of the components. This phenomenon is because some components may share the same receptor, thus causing an obstructive effect. This result may also be a reason that common bean is a nonhost of P. sojae. In addition, the relative local concentrations of citric acid in the root exudates of both nonhost cv. Yidianhong and resistant cv. Williams 82 were repellent to zoospores of P. sojae. Sloan amended with two concentrations of citric acids especially reduced zoospore chemoattraction, indicating that citric acid in the root exudates also plays an important role in host and nonhost resistance to P. sojae.
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影响因子：
3
作者：
Xu Bo-hen
通讯作者：
Xu Bo-hen
DOI：
--
发表时间：
2010-06
期刊：
--
影响因子：
--
作者：
Kara J. Riggs
通讯作者：
Kara J. Riggs
DOI：
10.1201/9780849384974-8
发表时间：
2007
期刊：
--
影响因子：
--
作者：
N. Uren
通讯作者：
N. Uren
DOI：
--
发表时间：
2010
期刊：
--
影响因子：
--
作者：
Hao WenYa;R. Wei;S. Qirong;Ren Lixuan
通讯作者：
Hao WenYa;R. Wei;S. Qirong;Ren Lixuan
影响因子：
7.4
作者：
GRAHAM, TL
通讯作者：
GRAHAM, TL