RAPID AGONIST MEDIATED PHOSPHORYLATION OF THE METABOTROPIC GLUTAMATE-RECEPTOR 1-ALPHA BY PROTEIN-KINASE-C IN PERMANENTLY TRANSFECTED BHK CELLS
RAPID AGONIST MEDIATED PHOSPHORYLATION OF THE METABOTROPIC GLUTAMATE-RECEPTOR 1-ALPHA BY PROTEIN-KINASE-C IN PERMANENTLY TRANSFECTED BHK CELLS
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DOI：
10.1016/0014-5793(95)00575-t
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发表时间：
1995-07-03
期刊：
影响因子：
3.5
通讯作者：
MCILHINNEY, RAJ
中科院分区：
文献类型：
--
作者：
ALALUF, S;MULVIHILL, ER;MCILHINNEY, RAJ
将与磷脂酶C偶联的代谢型谷氨酸受体1α(mGluR1α)永久性地转染入克隆BHK细胞，用(PO43-)-P-32标记、裂解，用特异性抗肽抗体进行免疫沉淀，用SDS-PAGE和放射自显影分析免疫沉淀物。受体被激动剂激活后，基础水平的受体磷酸化水平迅速和短暂地增加，这种激动剂效应具有剂量依赖性和快速的时间过程，并且可以被特异性的PKC抑制剂Ro318220所消除，这表明PKC参与了激动剂介导的受体的磷酸化。
Clonal BHK cells permanently transfected with the metabotropic glutamate receptor 1 alpha (mGluR1 alpha), which is coupled to phospholipase C, were used to study the phosphorylation state of the receptor, Cells were labelled with (PO43-)-P-32, lysed, the receptor immunoprecipitated with specific anti-peptide antibodies and the immunoprecipitates analysed by SDS-PAGE followed by autoradiography. A significant basal level of receptor phosphorylation was observed which was rapidly and transiently increased in response to agonist activation of the receptor, This agonist effect was found to be dose dependent with a rapid time course and could be abolished by the specific PKC inhibitor Ro318220, suggesting that PKC was responsible for the agonist mediated phosphorylation of the receptor.