Detection of Salmonella invA by isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) in Zambia
Detection of Salmonella invA by isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) in Zambia
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DOI：
10.1016/j.cimid.2005.10.001
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发表时间：
2005-09-01
影响因子：
2
通讯作者：
Yasuda, J
中科院分区：
文献类型：
--
作者：
Isogai, E;Makungu, C;Yasuda, J
等温嵌合引物启动的核酸扩增（isothermal and chimeric primer-initiated amplification of nucleic acids，ICAN）是由外切Bea DNA聚合酶、RNaseH和DNA-RNA嵌合引物组成的一种新的等温DNA扩增方法。我们从鸡尸体、蛋黄和牛粪便样品中检测到沙门氏菌的invA。ICAN-chromatostrip检测59株中有53株为invA阳性。结果与标准PCR方法一致。沙门氏菌invA检测12/14的胴体冲洗ICAN，而在7/14冲洗标准PCR。这些结果表明，ICAN是一个有效的，敏感的和简单的系统来检测沙门氏菌物种的invA在发展中国家，如赞比亚。(c)2005爱思唯尔有限公司保留所有权利。
The isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) is a new isothermal DNA amplification method composed of exo Bea DNA polymerase, RNaseH and DNA-RNA chimeric primers. We detected invA of Salmonella from chicken carcasses, egg yolk and cattle fecal samples. Fifty-three of 59 isolates were invA-positive in ICAN-chromatostrip detection. The result was consistent with those obtained by standard PCR. Salmonella invA was detected in 12 of 14 carcass rinses by ICAN, while in 7 of 14 rinses by standard PCR. These results indicate that ICAN is an efficient, sensitive and simple system to detect invA of Salmonella species in developing countries such as Zambia. (c) 2005 Elsevier Ltd. All rights reserved.