The Mannose-specific bulb lectin from Galanthus nivalis (snowdrop) binds mono- and dimannosides at distinct sites. Structure analysis of refined complexes at 2.3 angstrom and 3.0 angstrom resolution
The Mannose-specific bulb lectin from Galanthus nivalis (snowdrop) binds mono- and dimannosides at distinct sites. Structure analysis of refined complexes at 2.3 angstrom and 3.0 angstrom resolution
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DOI：
10.1006/jmbi.1996.0532
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发表时间：
1996-10-04
影响因子：
5.6
通讯作者：
Wright, CS
中科院分区：
文献类型：
--
作者：
Hester, G;Wright, CS
雪花莲凝集素（Galanthusnivalis agglutinin，GNA）是石蒜科植物鳞茎凝集素家族的一种甘露糖特异性凝集素。已经确定了与甲基-α-D-甘露糖（MeMan）和甘露糖-α 1，3-D-甘露糖-α-OMe（MeMan-2）复合的GNA的晶体结构，并在内部结构对称性和糖结合方面进行了分析。用0.167的X因子（所有数据）精制的2.29埃正交甲基-α-Man复合物的最终模型包括12个结合的糖配体和327个水分子。222四聚体的四个独立的亚基（A、B、C和D）和构成每个亚基的三个四链β-片层（I、II和III）比较接近（r.m.s.
Galanthus nivalis agglutinin (GNA, a 50 kDa tetramer) is a mannose-specific lectin of the Amaryllidaceae family of bulb lectins. Crystal structures of GNA complexed with methyl-alpha-D-mannose (MeMan) and mannose-alpha 1,3-D-mannose-alpha-OMe (MeMan-2) have been determined and analyzed in terms of internal structural symmetry and saccharide binding. The final model of the 2.29 Angstrom orthorhombic methyl-alpha-Man complex refined with an X-factor of 0.167 (all data) includes 12 bound sugar ligands and 327 water molecules. The four independent subunits (A, B, C and D) of the 222 tetramer and the three four-stranded beta-sheets (I,II and III) that constitute each subunit compare closely (r.m.s.Delta =