Ameloblast-lineage cells of rat tooth germs proliferate and scatter in response to hepatocyte growth factor in culture.
Ameloblast-lineage cells of rat tooth germs proliferate and scatter in response to hepatocyte growth factor in culture.
复制标题
大鼠牙胚的成釉细胞谱系细胞响应培养物中的肝细胞生长因子而增殖和分散。
DOI：
10.1387/ijdb.9879711
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
K. Kurisu
中科院分区：
文献类型：
--
作者：
T. Matsumura;M. Tabata;S. Wakisaka;M. Sakuda;K. Kurisu
肝细胞生长因子（HGF）被认为是早期器官发生过程中上皮 - 间质相互作用的介体之一，并参与鼠类磨牙的发展。在这项研究中，HGF及其受体C-MET的免疫组织化学定位显示，HGF分布在牙皮乳头中的增殖间充质细胞中，并且C-MET在大鼠发育中的上皮细胞中连续表达C-Met 。这些观察结果证实了HGF参与大鼠门牙的发展，如先前在磨牙中所示。然后，我们使用了由小鼠的下颌切牙制备的成熟细胞细胞的原发性培养，以检查HGF对成熟细胞生长和分化的直接影响。我们发现，在2-20 ng/mL时的HGF诱导了成成成木细胞的数量显着增加和此类细胞的散射。我们的结果表明，HGF同时促进了成熟细胞细胞的增殖和散射。
Hepatocyte growth factor (HGF) is considered to be one of the mediators of epithelial-mesenchymal interactions during early organogenesis and to be involved in the development of murine molars. In this study, the immunohistochemical localization of HGF and of its receptor, c-Met, revealed that HGF was distributed in the proliferating mesenchymal cells in the dental papillae and that c-Met was continuously expressed in the epithelial cells during the development of rat incisors. These observations confirmed the involvement of HGF in the development of rat incisors, as demonstrated previously in molars. We then used a primary culture of ameloblast-lineage cells, prepared from mandibular incisors of young rats, to examine the direct effects of HGF on the growth and differentiation of ameloblasts. We found that HGF at 2-20 ng/ml induced a marked increase in the number of ameloblast-lineage cells and in the scattering of such cells. Our results suggest that HGF promotes the proliferation and scattering of ameloblast-lineage cells simultaneously.