Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020
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DOI：
10.2807/1560-7917.es.2020.25.9.2000173
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发表时间：
2020-03-05
期刊：
影响因子：
19
通讯作者：
Sing, Andreas
中科院分区：
文献类型：
--
作者：
Konrad, Regina;Eberle, Ute;Sing, Andreas
当德国遭遇欧洲首次与旅行相关的严重急性呼吸系统综合征冠状病毒2 （SARS-CoV-2）疫情时，就出现了及时建立诊断方法的必要性。我们描述了我们在大型接触者追踪调查中的实验室经验，比较了以前发表的在不同PCR系统和商业试剂盒中的实时RT-PCR分析。我们发现，使用相同的引物和探针与不同的PCR系统的分析性能不同，商业试剂盒表现良好。
The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.