Proteomic analysis of Campylobacter jejuni 11168 biofilms reveals a role for the motility complex in biofilm formation
Proteomic analysis of Campylobacter jejuni 11168 biofilms reveals a role for the motility complex in biofilm formation
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DOI：
10.1128/jb.01975-05
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发表时间：
2006-06-01
影响因子：
3.2
通讯作者：
Szymanski, Christine M.
中科院分区：
文献类型：
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作者：
Kalmokoff, Martin;Lanthier, Patricia;Szymanski, Christine M.
在发达国家，空肠弯曲杆菌仍然是引起细菌性肠胃炎的主要原因，但人们对这种挑剔的有机体在其环境中生存的机制知之甚少。我们已经证明C空肠11168可以在各种表面形成生物膜。浮游和生物膜生长细胞的蛋白质组学分析表明，两种生长模式之间的蛋白质表达谱存在差异。参与运动复合物的蛋白质，包括鞭毛蛋白（FlaA, FlaB），丝帽（FliD），基体（FlgG, FlgG2）和趋化蛋白（CheA），在生物膜中的表达水平都高于在静止期浮游细胞中的表达水平。其他表达增强的蛋白包括参与一般（GroEL, GroES）和氧化（Tpx, Ahp）应激反应的蛋白，两种已知的粘附素（Peb1, FlaC），以及参与生物合成，能量产生和分解代谢功能的蛋白。鞭毛flhA突变体不仅失去了附着在固体基质上并形成生物膜的能力，而且不能在液体培养的气液界面上形成膜。影响鞭毛丝的基因（fliA,flaA,flaB,flaG）或细胞粘附素（flaC）的表达的插入失活也会导致鞭毛膜形成的延迟。这些发现表明，鞭毛运动复合物在空肠C. 11168在生物膜形成过程中与固体表面的初始附着以及在膜形成所需的细胞间相互作用中起着至关重要的作用。在成熟的生物膜中持续表达运动复合物是不寻常的，这表明鞭毛装置在生物膜表型中的作用。
Campylobacter jejuni remains the leading cause of bacterial gastroenteritis in developed countries, and yet little is known concerning the mechanisms by which this fastidious organism survives within its environment. We have demonstrated that C jejuni 11168 can form biofilms on a variety of surfaces. Proteomic analyses of planktonic and biofilm-grown cells demonstrated differences in protein expression profiles between the two growth modes. Proteins involved in the motility complex, including the flagellins (FlaA, FlaB), the filament cap (FliD), the basal body (FlgG, FlgG2), and the chemotactic protein (CheA), all exhibited higher levels of expression in biofilms than found in stationary-phase planktonic cells. Additional proteins with enhanced expression included those involved in the general (GroEL, GroES) and oxidative (Tpx, Ahp) stress responses, two known adhesins (Peb1, FlaC), and proteins involved in biosynthesis, energy generation, and catabolic functions. An aflagellate flhA mutant not only lost the ability to attach to a solid matrix and form a biofilm but could no longer form a pellicle at the air-liquid interface of a liquid culture. Insertional inactivation of genes that affect the flagellar filament (fliA,flaA,flaB,flaG) or the expression of the cell adhesin (flaC) also resulted in a delay in pellicle formation. These findings demonstrate that the flagellar motility complex plays a crucial role in the initial attachment of C. jejuni 11168 to solid surfaces during biofilm formation as well as in the cell-to-cell interactions required for pellicle formation. Continued expression of the motility complex in mature biofilms is unusual and suggests a role for the flagellar apparatus in the biofilm phenotype.