Blood pressure inversely correlates with thrombin-evoked calcium rise in platelets.
Blood pressure inversely correlates with thrombin-evoked calcium rise in platelets.
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血压与凝血酶引起的血小板钙升高呈负相关。
DOI：
10.1161/01.hyp.23.6.703
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Aviv,A
中科院分区：
文献类型：
--
作者：
Gardner,JP;Cho,JH;Skurnick,JH;Awad,G;Gutkin,M;Byrd,LH;Lasker,N;Aviv,A
早期的研究表明，人体血小板中的基础胞浆游离钙与血压呈正相关，然而，最近的研究未能显示这种关系。我们进行了目前的工作，以检查哪些血小板胞浆钙参数（即，在静息或刺激状态下的含钙和无钙介质中的胞浆钙）呈现最小的变异性和最好的相关性与血压。我们研究了17名健康的白色男性在三个不同的场合，间隔1周和4周。他们的手动和动态自动24小时血压测量与静息和刺激（凝血酶处理）fura 2-负载血小板的胞浆钙相关。在1 mmol/L钙和无钙培养基中测量以下胞质钙参数：基础胞质钙、凝血酶诱发的胞质钙峰值和凝血酶处理后5分钟的后瞬时胞质钙。在钙介质中，最高和最低的变异系数分别为基础胞浆钙（22.8%）和凝血酶诱导的峰值胞浆钙（10.1%）。基础胞浆钙与任何血压参数均不相关。在胞浆钙参数中，钙介质中凝血酶诱发的胞浆钙峰值与血压表现出一致的（负）相关性，自动和手动血压读数的舒张压比收缩压显示出更好的相关性。峰值凝血酶诱发的钙介质中的细胞溶质钙显示出类似的相关性与夜间和白天的自动血压测量。在无钙培养基中凝血酶诱发的胞浆钙峰值与血压之间无相关性。（250字处删节）
Earlier investigations showed a positive correlation between basal cytosolic free calcium in human platelets and blood pressure; however, recent studies have failed to show this relation. We undertook the present work to examine which platelet cytosolic calcium parameters (namely, cytosolic calcium in resting or stimulated states in calcium-containing and calcium-free media) present the least variability and best correlation with blood pressure. We studied 17 healthy white men on three different occasions separated by 1- and 4-week intervals. Their manual and ambulatory automated 24-hour blood pressure measurements were correlated with cytosolic calcium in resting and stimulated (thrombin-treated) fura 2-loaded platelets. The following cytosolic calcium parameters were measured in 1 mmol/L calcium and calcium-free media: basal cytosolic calcium, peak thrombin-evoked cytosolic calcium, and post-transient cytosolic calcium 5 minutes after thrombin treatment. The highest and lowest coefficients of variation were respectively shown by the basal cytosolic calcium (22.8%) and peak thrombin-evoked cytosolic calcium (10.1%) in calcium medium. Basal cytosolic calcium did not correlate with any of the blood pressure parameters. Of the cytosolic calcium parameters, peak thrombin-evoked cytosolic calcium in calcium medium demonstrated consistent (negative) correlations with blood pressure, with better correlations shown with diastolic than systolic blood pressure of both automated and manual blood pressure readings. Peak thrombin-evoked cytosolic calcium in calcium medium showed similar correlations with nighttime and daytime automated blood pressure measurements. There were no correlations between peak thrombin-evoked cytosolic calcium in calcium-free medium and blood pressure.(ABSTRACT TRUNCATED AT 250 WORDS)