Location of sugars in barley seeds during germination by NMR microscopy
Location of sugars in barley seeds during germination by NMR microscopy
复制标题
通过核磁共振显微镜观察发芽过程中大麦种子中糖的位置
DOI：
10.1111/j.1365-3040.1996.tb00020.x
复制
发表时间：
1996
影响因子：
7.3
通讯作者：
H. Kano
中科院分区：
文献类型：
--
作者：
N. Ishida;M. Koizumi;H. Kano
利用~（13）C核磁共振（NMR）谱、~ 1H-NMR成像和~ 1H-NMR定域谱研究了大麦种子萌发过程中糖的分布和变化。用~（13）C-NMR谱分析了吸胀种子中的麦芽糖、蔗糖、果糖和油脂。在发芽的第一个6天，麦芽糖含量增加，油含量逐渐下降，而蔗糖和果糖的水平保持不变。糖的1H-NMR图像和1H-NMR定位光谱定位在维管束的种子，以及在可溶性胚乳。它们也在芽中被检测到。在80%乙醇提取物中检测到的糖是蔗糖和葡萄糖，它们位于维管束中，而不是在胚芽鞘的叶肉细胞中。它们也位于茎的基部，但不超过7毫米的小盾。这些数据表明，糖主要是通过维管束运输，并在同一时间，迅速纳入叶肉细胞在叶片中。
The distribution and fluctuation of sugars in germinating barley seeds were examined by 13C nuclear magnetic resonance (NMR) spectroscopy, 1H-NMR imaging and 1H-NMR localized spectroscopy in relation to morphology. Maltose, sucrose, fructose and oils were detected in intact imbibed seeds by 13C-NMR spectra. During the first 6 d of germination, the maltose content increased and the oil content gradually decreased, whilst the levels of sucrose and fructose remained constant. Sugars were located by 1H-NMR images and 1H-NMR localized spectra in the vascular bundle of the seeds as well as in the solubilized endosperm. They were also detected in the shoots. The sugars detected in an 80% ethanol shoot extract were sucrose and glucose, which were located in the vascular bundles but not in the mesophyll cells of the coleoptile. They were also located in the basal part of the shoot, but not above 7 mm from the scutellum. The data suggest that the sugars are primarily transported through the vascular bundles and, at the same time, rapidly incorporated into mesophyll cells in the leaves.